Dataset Information

Function of two GSA isoforms in Arabidopsis thaliana

ABSTRACT: As proof of interdependent interaction between GSAT, GluTR, and GBP, a crosslinking mass spectrometry experiment was conducted with bilateral and trilateral combinations of the three respective recombinant proteins. Apart from the known GBP – GluTR interaction, combined assays for mutual interaction of GSAT either with GluTR or GBP were carried out and ultimately a crosslinking experiment was performed including all three proteins. The proteins of these assays were subsequently cleaved by trypsin and analysed by mass spectrometry to identify the crosslinked peptides of the three proteins in all possible of dual and triple combinations. This approach enabled the detection of the interrelation between all three proteins.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Bernhard Grimm

PROVIDER: PXD029358 | JPOST Repository | Wed Nov 16 00:00:00 GMT 2022

REPOSITORIES: jPOST

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			Action	DRS
	200227_C20_004_QHF1_PTM_Rev01_%2320_013_PAP343.raw	Raw
	200227_C20_004_QHF1_PTM_Rev01_%2320_013_PAP344.raw	Raw
	200227_C20_004_QHF1_PTM_Rev01_%2320_013_PAP345.raw	Raw
	200227_C20_004_QHF1_PTM_Rev01_%2320_013_PAP346.raw	Raw
	200227_C20_004_QHF1_PTM_Rev01_%2320_013_PAP347.raw	Raw

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Publications

Glutamate 1-semialdehyde aminotransferase is connected to GluTR by GluTR-binding protein and contributes to the rate-limiting step of 5-aminolevulinic acid synthesis.

Sinha Neha N Eirich Jürgen J Finkemeier Iris I Grimm Bernhard B

The Plant cell 20221001 11

Tetrapyrroles play fundamental roles in crucial processes including photosynthesis, respiration, and catalysis. In plants, 5-aminolevulinic acid (ALA) is the common precursor of tetrapyrroles. ALA is synthesized from activated glutamate by the enzymes glutamyl-tRNA reductase (GluTR) and glutamate-1-semialdehyde aminotransferase (GSAAT). ALA synthesis is recognized as the rate-limiting step in this pathway. We aimed to explore the contribution of GSAAT to the control of ALA synthesis and the form ...[more]

PMID: 35972388

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