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CSF exosome proteome of Alzheimer's disease

ABSTRACT: Exosome fractions were purified from spinal fluid collected from Alzheimer's disease patients and control subjects using MagCapture. Elution of exosomes bound to affinity beads was performed with 1% SDS. Proteins were precipitated from the exosome fraction by acetone precipitation; the precipitates were dissolved in 50% trifluoroethanol and subjected to reduction with DTT and alkylation reaction with iodoacetamide. The proteins were degraded by trypsin/Lys-C, and the peptides produced were desalted and purified using MonoSpinC18. The peptides were measured by LC-MS/MS and quantified by non-label quantification method. The peak area values of peptide-proteins for each sample were normalized to the total peak area.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Tatsuo Mano

PROVIDER: PXD029789 | JPOST Repository | Sat Jan 06 00:00:00 GMT 2024

REPOSITORIES: jPOST

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			Action	DRS
	211102_Sato_IDA_37.wiff	Wiff
	211102_Sato_IDA_37.wiff.scan	Wiff
	211102_Sato_IDA_38.wiff	Wiff
	211102_Sato_IDA_38.wiff.scan	Wiff
	211102_Sato_IDA_39.wiff	Wiff

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Exosome of plasma and CSF from AD patients

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