Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MS-based arginine monomethylation study in Jurkat PRMT7 KO cells

ABSTRACT: Effects of Prmt7 genetic deletion to arginine monomethylation in Jurkat T-ALL cell line. Analysis was made using two different Prmt7 KO and one control cell lines.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Olli Lohi

PROVIDER: PXD032819 | JPOST Repository | Mon Mar 28 00:00:00 BST 2022

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	abundances_all_samples.msf	Msf
	set1_CTRL_EV_1.raw	Raw
	set1_CTRL_EV_2.raw	Raw
	set1_CTRL_EV_3.raw	Raw
	set1_PRMT7_34_1.raw	Raw

Items per page:

1 - 5 of 19

Publications

Arginine Methyltransferase PRMT7 Deregulates Expression of RUNX1 Target Genes in T-Cell Acute Lymphoblastic Leukemia.

Oksa Laura L Mäkinen Artturi A Nikkilä Atte A Hyvärinen Noora N Laukkanen Saara S Rokka Anne A Haapaniemi Pekka P Seki Masafumi M Takita Junko J Kauko Otto O Heinäniemi Merja M Lohi Olli O

Cancers 20220426 9

T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy with no well-established prognostic biomarkers. We examined the expression of protein arginine methyltransferases across hematological malignancies and discovered high levels of <i>PRMT7</i> mRNA in T-ALL, particularly in the mature subtypes of T-ALL. The genetic deletion of <i>PRMT7</i> by CRISPR-Cas9 reduced the colony formation of T-ALL cells and changed arginine monomethylation patterns in protein complexes ...[more]

PMID: 35565298

Similar Datasets

KO-2806 Monotherapy and Combination Therapies in Advanced Solid Tumors

Project description:This first-in-human (FIH) dose-escalation and dose-validation/expansion study will assess KO-2806, a farnesyl transferase inhibitor (FTI), as a monotherapy and in combination, in adult patients with advanced solid tumors.

| 91122 | ecrin-mdr-crc

Protein profile in a panel of HEK293A cell lines with genetic deletion

Project description:MS Analysis of genetic KO in a panel of HEK293A cell lines with genetic deletion

2023-03-04 | PXD030368 | Pride

RNA expression data from hepatocytes from WT, NemoLPC-KO, Cxcr6-/- and NemoLPC-KOCxcr6-/- mice

Project description:We investigated the role of CXCR6 in the NEMOLPC-KO mouse model. Genetic deletion of CXCR6 enhanced hepatocyte death, inflammation and fibrosis in NEMOLPC-KO mice. This study aimed at characterizing the transcriptomic changes in hepatocytes from these mice compared to controls.

2018-12-07 | GSE121124 | GEO

Decreased DEPTOR expression is involved in enhanced cell motility and invasion capability of ASS1 (Argininosuccinate synthetase 1) -KO cells

Project description:To elucidate the mechanisms underlying the enhanced sensitivity to arginine in the absence of ASS1 expression in endometrial cancer cells, we performed DNA microarray-based gene expression profiling using HEC1B harboring the empty vector (EV) and ASS1-knockout (KO) HEC1B cells. Since accumulating evidence has shown that arginine regulates mTORC1 activity, we focused on the expression patterns of genes related to the mTOR complex. ASS1-KO HEC1B cells had lower DEPTOR transcript abundance than EV cells, while no significant difference was observed in the expression levels of genes coding for other mTOR complex components such as mTOR, RAPTOR, or RICTOR. Indeed, immunoblot analysis confirmed that the expression level of DEPTOR protein was significantly decreased in ASS1-KO HEC1B cells, which were cultured in arginine-replete conditions.

2017-04-02 | GSE86963 | GEO

mRNA seq of MOSAICS based Irs1 KO, Srebf1 KO, Tbx3 KO, Bcl6 KO, Smyd2 KO and control (sgGFP and sgLacZ) livers

Project description:To investigate expression changes of the KO vs. control livers under 3 months of Western Diet. We then performed mRNA seq analysis on MOSAICS based single-gene whole-liver KO samples and control samples

2024-09-01 | GSE212916 | GEO

Gene Expression Analysis of RBM5 KO in Brain

Project description:This study analyzed the effect of RBM5 gene deletion in cortical brain tissue on differential gene expression/splicing changes 48h after a traumatic brain injury (TBI). TBI was induced in WT vs. KO mice by controlled cortical impact (CCI) injury. The four grouops included: (1) Sham-WT, (2) CCI-WT, (3) Sham-KO, and (4) CCI-KO. The objective of this study was to test if RBM5 KO decreased the expression of cell death mediators in the contused brain 48h post-injury.

2021-05-12 | GSE128543 | GEO

Gene expression profiling of WT or Dicer KO iNKT cells and T cells

Project description:iNKT cells are a T lymphocyte subset displaying an innate effector phenotype that is acquired through a thymic developmental program controlled by microRNAs (miRNAs). iNKT cells lacking all miRNAs by the deletion of Dicer (Dicer KO) are markedly reduced and display a complete maturation block. In this study, we sought to gain insight into the miRNA-regulated genetic program required for iNKT cell development. By systemic analysis, we identified transcripts differentially expressed between thymic WT or Dicer KO iNKT cells and targeted by the iNKT cell-specific miRNAs. TGF-βRII, a molecule critically implicated in iNKT cell maturation, was found upregulated in Dicer KO iNKT cells together with increased TGF-β-dependent signaling. miRNAs belonging to the paralog miR-106a~363, miR-106b~25 and miR-17~92 clusters were predicted to target TGF-βRII mRNA during iNKT cell development. Thymic iNKT cells lacking all three miRNA clusters displayed both increased TGF-βRII expression and signaling and a maturation block, recapitulating those found in Dicer KO iNKT cells. Consistently, inhibition of TGF-β-dependent signaling in the absence of miRNAs, by crossing TGF-βRII KO and Dicer KO mice, rescued iNKT cell maturation. Collectively, our results highlight a fundamental requirement of the modulation of TGF-β-dependent signaling by miRNAs for iNKT cell development

2016-11-17 | GSE79901 | GEO

Transcriptome profiles of hematopoietic lineages from BAP1 WT and KO mice

Project description:BAP1 has been studied as a tumor suppressor. Our aim was to characterize genes that were altered in various hematopoietic cell types upon deletion of BAP1. BAP1 WT versus KO cells.

2012-11-07 | E-GEOD-40541 | biostudies-arrayexpress

Genome-wide sequencing of cultured MDSCs obtained from bone marrow of lnc57Rik ko , lncGM1082 ko and WT mice.

Project description:To fully elucidate the effects of long non-coding RNA 5730403I07Rik (lnc57Rik) and lncGM1082 on myeloid-derived suppressor cell (MDSCs), we generated lnc57Rik knockout (KO) and lncGM1082 ko mice. Then bone marrow cells were obtained from the femurs of C57BL/6, lnc57Rik KO, or lncGM1082 ko mice and cultured in RPMI-1640 medium supplemented with GM-CSF plus IL6 for 4 days. We further performed a high-throughput sequencing analysis in cultured MDSCs obtained from bone marrow of lnc57Rik ko, lncGM1082 ko and wild type (WT) mice.

2022-03-03 | GSE197566 | GEO

Aortic samples of inducible vascular smooth muscle specific KO of YAP/TAZ

Project description:YAP and TAZ are transcriptional co-activators and downstream effectors of the Hippo pathway, which play crucial roles in organ size control and cancer pathogenesis. Genetic deletion of YAP/TAZ has revealed their critical importance for embryonic development of the heart, vasculature and gastrointestinal mesenchyme. The aim of this study was to determine the long term role of YAP/TAZ in adult vascular smooth muscle in vivo. We used the novel Itga8-CreERT2 mouse for deletion of YAP/TAZ (i8-Y/T-KO).

2023-08-13 | GSE240233 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data