Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of proteome profiles between MDCK cells and SI-6 cells post infection for influenza virus A/Panama/2007/1999(H3N2)

ABSTRACT: Our recent data indicates that SI-6 cells, which is derived from MDCK cells, possess higher yield of influenza viruses than MDCK cells after influenza virus infection. However, it is not clear why SI-6 cells show high efficiency for virus production. To reveal the molecular basis for this phenomenon, we performed iTRAQ quantitative proteome analysis between MDCK cells and SI-6 cells post infection for influenza virus.

ORGANISM(S): Canis Lupus

SUBMITTER: Shigehiro Sato

PROVIDER: PXD036375 | JPOST Repository | Tue Aug 29 00:00:00 BST 2023

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Canis%20lupus%20familiaris%20&%20Influenza%20A%20virus%20(A,Panama,2007,1999(H3N2)).xlsx	Xlsx
	OFFGEL_fraction_1.raw	Raw
	OFFGEL_fraction_2.raw	Raw
	OFFGEL_fraction_3.raw	Raw
	OFFGEL_fraction_4.raw	Raw

Items per page:

1 - 5 of 7

Similar Datasets

Transcriptional response of Drosophila cells to FHV infection (infection experiment)

2009-04-01 | GSE15466 | GEO

Transcription profiling of bovine dendritic cells after infection with virulent or avirulent <br>rinderpest viruses., measles virus or mock infection

Project description:Changes in transcription in bovine dendritic cells after infection with virulent or avirulent <br>rinderpest viruses., measles virus or mock infection.

2009-12-07 | E-MEXP-1856 | biostudies-arrayexpress

Transcriptional response of Drosophila cells to FHV infection (infection experiment)

Project description:Virus infections induce cellular gene up and down regulation, and these changes often provide clues to cellular pathways utilized by viruses. We used microarrays to examine the transcriptional responses of cultured Drosophila S2 cells to infection with Flock House virus (FHV). Experiment Overall Design: Cultured S2 cells were infected with FHV at an MOI of 10 and we measured global transcript levels at 12 h after infection compared to control mock infected cells using Affymetrix Drosophila Genome 1.0 microarray chips.

2009-04-11 | E-GEOD-15466 | biostudies-arrayexpress

Global murine neutrophil response to highly and less virulent influenza A (H3N2) virus infections at 3 and 9 h post-infection

Project description:Array analysis of total RNA obtained from MPRO neutrophils collected at 3 and 9 h post-infection with highly and less virulent influenza A (H3N2) viruses. Viruses (designated as LVI, HVI and MPI) were derived from influenza strain virus A/Aichi/2/68 (Aichi68). LVI is Aichi68 propagated in eggs, HVI is mouse adapted Aichi68, and MPI is HVI propagated in MDCK. Infection: lung homogenate (mock), LVI, HVI and MPI; time of sample collection: 3 and 9 h post-infection; two biological replicates for each group.

2014-03-19 | E-GEOD-55995 | biostudies-arrayexpress

Global murine pulmonary response to highly and less virulent influenza A (H3N2) virus infections at 12, 48 and 96 h post-infection

Project description:Array analysis of total lung RNAs from female BALB/c mice collected at 12, 48 and 96 h post-infection with highly and less virulent influenza A (H3N2) viruses. Viruses (designated as LVI and HVI) were derived from influenza strain virus A/Aichi/2/68 (Aichi68). LVI is Aichi68 propagated in eggs, and HVI is mouse adapted Aichi68. Infection: lung homogenate (mock), LVI and HVI; time of sample collection: 12, 48 and 96 h post-infection; two biological replicates for each group.

2014-03-19 | E-GEOD-55994 | biostudies-arrayexpress

Infection of MK2 cells with monkeypox virus

Project description:Orthopox viruses, including monkeypox, multiply intracellularly and induce numerous changes in host genes expression. The virus target mainly humoral host response, and simultaneously, exploits other genes and functions to reproduce effectively. The goal of this experiment is to identify those host genes and functions that are essential for monkeypox virus replication. Mock infected control cells were treated and incubated identically to time point arms, except for virus exposure. Two time points of cells infected with monkeypox virus were harvested at 3, 7 hours post infection, and gene expression was assessed using microarray in all arms. The experiment was done in triplicate.

2010-04-07 | E-GEOD-21001 | biostudies-arrayexpress

Gene expression in Calu-3 cells after influenza H3N2 and H11N9 infection

Project description:Airway epithelial cells are the initial site of infection with influenza viruses. The innate immune responses of airway epithelial cells to infection have the potential to limit virus replication and induce effective adaptive immune responses. However, relatively little is known about the importance of this innate anti-viral response to infection. Avian influenza viruses are a potential source of future pandemics, therefore it is critical to examine the effectiveness of the host anti-viral system to different influenza viruses. We used a human influenza (H3N2) and a low pathogenic avian influenza (H11N9) to assess and compare the anti-viral responses of bronchial epithelial cells (BECs). After infection, the H3N2 virus replicated more effectively than the H11N9 strain in BECs. This was not due to differential expression of different sialic acid residues on BECs but was attributed to the interference of the host anti-viral responses by H3N2. The H3N2 strain induced a delay in anti-viral signaling and impaired release of type I and type III interferons (IFNs) compared to the H11N9 virus. We then transfected the gene encoding for non-structural (NS) 1 protein into the BECs and the H3N2 NS1 induced a greater inhibition of anti-viral responses compared to the H11N9 NS1. While the low pathogenic avian influenza virus was capable of infecting BECs, the human influenza virus replicated more effectively than avian influenza virus in BECs and this may be at least in part due to a differential ability of the two NS1 proteins to inhibit anti-viral responses. This suggests that the subversion of human anti-viral responses may be an important requirement for influenza viruses to adapt to the human host and induce disease.

2009-12-19 | GSE19580 | GEO

Global murine pulmonary response to highly and less virulent influenza A (H3N2) virus infections at 12, 48 and 96 h post-infection

2014-03-19 | GSE55994 | GEO

A Systems Approach to Understanding Human Rhinovirus and Influenza Virus Infection

Project description:Human rhinovirus and influenza virus infections of the upper airway lead to colds and the flu and can trigger exacerbations of lower airway diseases including asthma and chronic obstructive pulmonary disease. Despite modest advances in the diagnosis and treatment of infections by these viruses, novel diagnostic and therapeutic targets are still needed to differentiate between the cold and the flu, since the clinical course of influenza can be severe while that of rhinovirus is usually more mild. In our investigation of influenza and rhinovirus infection of human respiratory epithelial cells, we used a systems approach to identify the temporally changing patterns of host gene expression from these viruses. After infection of human bronchial epithelial cells (BEAS-2B) with rhinovirus, influenza virus or co-infection with both viruses, we studied the time-course of host gene expression changes over three days. From these data, we constructed a transcriptional regulatory network model that revealed shared and unique host responses to these viral infections such that after a lag of 4-8 hours, most cell host responses were similar for both viruses, while divergent host cell responses appeared after 24-48 hours. The similarities and differences in gene expression after epithelial infection of rhinovirus, influenza virus, or both viruses together revealed qualitative and quantitative differences in innate immune activation and regulation. These differences help explain the generally mild outcome of rhinovirus infections compared to influenza infections which can be much more severe. Human bronchial epithelial cells (BEAS-2B) were infected with rhinovirus, influenza virus or both viruses and RNAs were then profiled at 10 time points (2, 4, 6, 8, 12, 24, 26, 48, 60 and 72hrs)

2015-12-21 | E-GEOD-71766 | biostudies-arrayexpress

Global murine neutrophil response to highly and less virulent influenza A (H3N2) virus infections at 3 and 9 h post-infection

2014-03-19 | GSE55995 | GEO