Dataset Information

TCR phosphoproteome using DP thymocytes stimulated by H2-Kb MHC tetramers

ABSTRACT: Quantitative phosphoproteome with OT-I TAP-/- mice DP thymocytes stimulated by H2-Kb tetramers for 2 min. The quantitative data were calculated by the spike-in standard lysates from DPK cells cultured in the heavy media containing 15N2 13C6-lysine and 15N4 13C6-arginine.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Keiichi Nakayama

PROVIDER: PXD036851 | JPOST Repository | Thu Jul 27 00:00:00 BST 2023

REPOSITORIES: jPOST

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	orb_160422_A-SF_Q4H7_%231.raw	Raw
	orb_160422_A-SF_Q4H7_%232.raw	Raw
	orb_160422_A-SF_Q4H7_%233.raw	Raw
	orb_160422_A-SF_Q4H7_%234.raw	Raw
	orb_160422_A-SF_Q4H7_%235.raw	Raw

Items per page:

1 - 5 of 20

Similar Datasets

Project description:Protein extraction and proteolytic digestion were performed using a Filter-Assisted Sample Preparation (FASP) protocol. In case of phosphopeptide enrichment immobilized Fe(III) affinity chromatography (Fe-IMAC) was performed. The peptides were fractionated using an HPLC system fitted with an SCX column. 10 sample datasets were used: 1. CRC_N: Normal tissue samples were obtained from 20 colorectal cancer patients, no quantification.Phosphopeptide enrichment was performed. 2. CRC_T: Human colorectal cancer tissue samples were obtained from 20 patients, no quantification. Phosphopeptide enrichment was performed. 3. CRC_iTRAQ: Human colorectal cancer tissue samples were obtained from 24 patients, iTRAQ quantification. 4. CRC_phospho_iTRAQ:Human colorectal cancer tissue samples were obtained from 24 patients, iTRAQ quantification.Phosphopeptide enrichment was performed. 5. HCT116_iTRAQ: Human colon cancer cell HCT116, iTRAQ quantification. 6. HCT116_phospho_iTRAQ: Human colon cancer cell HCT116, phosphopeptide enrichment, iTRAQ quantification. 7. SW_SILAC_HL: Human colon cancer cell SW480 and SW620, SW480 and SW620 were grown in SILAC media, containing l-arginine (Arg0) and l-lysine (Lys0) (SW480; light), or 13C615N4-l-arginine (Arg10) and 13C6-l-Lysine (Lys6) (SW620; heavy). 8. SW_SILAC_LH: SW480 and SW620 were grown in SILAC media, containing l-arginine (Arg0) and l-lysine (Lys0) (SW620; light), or 13C615N4-l-arginine (Arg10) and 13C6-l-Lysine (Lys6) (SW480; heavy). 9. SW_phospho_SILAC_HL: SW480 and SW620 were grown in SILAC media, containing l-arginine (Arg0) and l-lysine (Lys0) (SW480; light), or 13C615N4-l-arginine (Arg10) and 13C6-l-Lysine (Lys6) (SW620; heavy). Phosphopeptide enrichment was performed. 10. SW_phospho_SILAC_LH: SW480 and SW620 were grown in SILAC media, containing l-arginine (Arg0) and l-lysine (Lys0) (SW620; light), or 13C615N4-l-arginine (Arg10) and 13C6-l-Lysine (Lys6) (SW480; heavy). Phosphopeptide enrichment was performed. For creation of xml and mgf files, Proteome Discoverer 1.3 and Mascot v2.3 software were used.

Dataset Information

TCR phosphoproteome using DP thymocytes stimulated by H2-Kb MHC tetramers

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets