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Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro

ABSTRACT: In mammalian testes, premeiotic spermatogonia respond to retinoic acid by completing an essential lengthy differentiation program before initiating meiosis. The molecular and cellular changes directing these developmental processes remain largely undefined. This wide gap in knowledge is due to two critical unresolved technical challenges: (1) lack of robust and reliable in vitro models to study differentiation and meiotic initiation; and (2) lack of methods to isolate large and pure populations of male germ cells at each stage of differentiation and at meiotic initiation. Here, we report a facile in vitro differentiation and meiotic initiation system that can be readily manipulated, including the use of chemical agents that cannot be safely administered to live animals. In addition, we present a transgenic mouse model enabling fluorescence-activated cell sorting-based isolation of millions of spermatogonia at specific developmental stages as well as meiotic spermatocytes

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Geyer, Christopher

PROVIDER: PXD037781 | JPOST Repository | Fri Oct 28 00:00:00 BST 2022

REPOSITORIES: jPOST

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			Action	DRS
	2021.08.11_TBresults_10.05.20_vs_+RAresults_05.05.21.mzML	Mzml
	2021.08.11_TBresults_10.05.20_vs_+RAresults_05.05.21.mzTab	Mztab
	GS4.raw	Raw
	GS5.raw	Raw
	GS6.raw	Raw

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