Proteomics

Dataset Information

0

Simultaneous proteome localization and turnover analysis reveals spatiotemporal coordinations of unfolded protein response

ABSTRACT: The functionality of proteins is dependent on their spatial and temporal distributions, neither of which is directly measured by static protein abundance. Here we report a mass spectrometry-based proteomics workflow and data analysis pipeline, named Simultaneous Proteome Localization and Turnover (SPLAT), to concurrently examine the turnover dynamics and subcellular distributions of whole cell proteomes under perturbation. SPLAT builds on prior work in protein turnover measurements and subcellular localization profiling, by combining dynamic stable isotope labeling, differential ultracentrifugation, and kinetic modeling to concurrently measure changes in protein turnover and subcellular localization under perturbation in one experiment.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Edward Lau 

PROVIDER: PXD038054 | JPOST Repository | Sat Dec 31 00:00:00 GMT 2022

REPOSITORIES: jPOST

Json Xml

Dataset's files

Source:
Action DRS
20220725_JC_SILAC_LOPIT_CTRL_F01.raw Raw
20220725_JC_SILAC_LOPIT_CTRL_F13.raw Raw
20220725_JC_SILAC_LOPIT_CTRL_F14.raw Raw
20220725_JC_SILAC_LOPIT_CTRL_F15.raw Raw
20220725_JC_SILAC_LOPIT_CTRL_F16.raw Raw
Items per page:
1 - 5 of 20
altmetric image

Publications

Simultaneous proteome localization and turnover analysis reveals spatiotemporal features of protein homeostasis disruptions.

Currie Jordan J   Manda Vyshnavi V   Robinson Sean K SK   Lai Celine C   Agnihotri Vertica V   Hidalgo Veronica V   Ludwig R W RW   Zhang Kai K   Pavelka Jay J   Wang Zhao V ZV   Rhee June-Wha JW   Lam Maggie P Y MPY   Lau Edward E  

Nature communications 20240311 1

The spatial and temporal distributions of proteins are critical to protein function, but cannot be directly assessed by measuring protein bundance. Here we describe a mass spectrometry-based proteomics strategy, Simultaneous Proteome Localization and Turnover (SPLAT), to measure concurrently protein turnover rates and subcellular localization in the same experiment. Applying the method, we find that unfolded protein response (UPR) has different effects on protein turnover dependent on their subc  ...[more]

Similar Datasets

Proteomics Simultaneous Proteome Localization and Turnover Analysis, MS3 Data
2023-11-08 | PXD046669 | JPOST Repository
Proteomics Simultaneous proteome localization and turnover analysis, Rep. 3 (Corrected)
2023-11-08 | PXD046671 | JPOST Repository
Proteomics SubCellBarcode: Scalable proteome-wide mapping of protein localization and relocalization
2021-02-17 | MSV000086899 | MassIVE
Proteomics SubCellBarcode: Scalable proteome-wide mapping of protein localization and relocalization
2019-01-03 | PXD006895 | Pride
Transcriptomics Atlas of subcellular RNA localization in human embryonic stem cells and its flow during differentiation
2023-12-22 | GSE206328 | GEO
Transcriptomics Global organelle profiling reveals subcellular localization and remodeling at proteome scale
2024-10-04 | GSE278059 | GEO
Genomics Homo sapiens
| PRJNA481540 | ENA
Genomics GAR-Driven Subcellular Localization of the Growth Controlling RNA Binding Protein Nucleolin
| PRJNA597482 | ENA
Transcriptomics Regulation of RNA localization by FMR1
2020-05-28 | GSE137878 | GEO
Transcriptomics RNA localization to protrusions of mouse melanoma YUMM1.7 cells.
2023-02-24 | GSE219089 | GEO