Dataset Information

Proteome analysis of neonatal mouse brain and brain capillaries

ABSTRACT: The functions and protein expressions of the blood-brain barrier are changed during brain development after birth. The purpose of the present study was to develop a method to isolate brain capillaries from a single frozen neonatal mouse brain and elucidate the enrichment of brain capillaries by quantitative proteomic analysis. The brain capillary fraction was prepared by the optimized method from a single frozen mouse neonatal brain (postnatal day 7). The brain capillary fractions and brain lysates were digested by trypsin and analyzed by DIA.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Sumio Ohtsuki

PROVIDER: PXD039296 | JPOST Repository | Wed Jul 12 00:00:00 BST 2023

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	Data220615_B-cap_WT_1.wiff	Wiff
	Data220615_B-cap_WT_1.wiff.scan	Wiff
	Data220615_B-cap_WT_2.wiff	Wiff
	Data220615_B-cap_WT_2.wiff.scan	Wiff
	Data220615_B-cap_WT_3.wiff	Wiff

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Publications

Development of a method for isolating brain capillaries from a single neonatal mouse brain and comparison of proteomic profiles between neonatal and adult brain capillaries.

Hamada Yudai Y Ogata Seiryo S Masuda Takeshi T Ito Shingo S Ohtsuki Sumio S

Fluids and barriers of the CNS 20230623 1

<h4>Background</h4>The functions and protein expressions of the blood-brain barrier are changed throughout brain development following birth. This study aimed to develop a method to isolate brain capillaries from a single frozen neonatal mouse brain and elucidate the enrichment of brain capillaries by quantitative proteomic analysis. We further compared the expression profile of proteins between neonatal and adult brain capillary fractions.<h4>Methods</h4>The brain capillary fraction was prepare ...[more]

PMID: 37353852

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