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Serum proteome of dogs with precursor-targeted immune-mediated anemia before and after splenectomy

ABSTRACT: There have been clinical reports of splenectomy as a treatment option for immune-mediated hematologic diseases in humans and dogs. We focused on the efficacy of splenectomy as a treatment for dogs with precursor-targeted immune-mediated anemia. We collected serum samples of pre and post splenectomy in these dogs to investigate the factors that might improve non-regenerative anemia after splenectomy.

ORGANISM(S): Cellular Organisms Canis Lupus Familiaris

SUBMITTER: Mei Sugawara-Suda

PROVIDER: PXD039913 | JPOST Repository | Tue Feb 28 00:00:00 GMT 2023

REPOSITORIES: jPOST

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			Action	DRS
	Dog_KK0817-1_PIMA1_pre.raw	Raw
	Dog_KK0817-2_PIMA1_post.raw	Raw
	Dog_KK0817-3_PIMA2_pre.raw	Raw
	Dog_KK0817-4_PIMA2_post.raw	Raw
	Dog_KK1087-1_PIMA3_pre.raw	Raw

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Project description:T-cells play a crucial role in canine immunoregulation and defence against invading pathogens. Proliferation is fundamental to T-cell differentiation, homeostasis and immune response. Initiation of proliferation following receptor mediated stimuli requires a temporally programmed gene response that can be identified as immediate-early, mid- and late phases. The immediate-early response genes in T-cell activation engage the cell cycle machinery and promote subsequent gene activation events. Genes involved in this immediate-early response in dogs are yet to be identified. The present study was undertaken to characterize the early T-cell gene response in dogs to improve understanding of the genetic mechanisms regulating immune function. Gene expression profiles were characterized using canine gene expression microarrays and quantitative reverse transcription PCR (qRT-PCR), and paired samples from eleven dogs. Significant functional annotation clusters were identified following stimulation with phytohemagluttinin (PHA) (5μg/ml), including the Toll-like receptor signaling pathway and phosphorylation pathways. Using strict statistical criteria, 13 individual genes were found to be differentially expressed, nine of which have ontologies that relate to proliferation and cell cycle control. These included, prostaglandin-endoperoxide synthase 2 (PTGS2/COX2), early growth response 1 (EGR1), growth arrest and DNA damage-inducible gene (GADD45B), phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1), V-FOS FBJ murine osteosarcoma viral oncogene homolog (FOS), early growth response 2 (EGR2), hemogen (HEMGN), polo-like kinase 2 (PLK2) and polo-like kinase 3 (PLK3). Differential gene expression was re-examined using qRT-PCR, which confirmed that EGR1, EGR2, PMAIP1, PTGS2, FOS and GADD45B were significantly upregulated in stimulated cells and ALAS2 downregulated. PTGS2 and EGR1 showed the highest levels of response in these dogs. Both of these genes are involved in cell cycle regulation. This study provides a comprehensive analysis of the early T-cell gene response to activation in dogs.

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Serum proteome of dogs with precursor-targeted immune-mediated anemia before and after splenectomy

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