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Direct phosphorylation of SIZ1 by SnRK2s kinase to response the osmotic stress in Arabidopsis

ABSTRACT: SUMOylation is an essential and highly dynamic post-translational modification that regulates developmental processes and stress adaptations in plants to environmental cues. The global SUMOylation is quickly induced by dehydration and hyperosmotic stresses in plants, while the detailed mechanism underlying such SUMOylation dynamics is largely unknown. Herein, we report key components in osmotic stress and abscisic acid signaling, SNF1-related protein kinase 2s (SnRK2s), phosphorylate SUMO E3 ligase SAP and MIZ1-1 (SIZ1) to stabilize SIZ1 protein under stress conditions. The Ser820 residue is a functional SnRK2 phosphosite and non-phosphorylatable SIZ1S820A is unstable in vivo and in vitro. Under osmotic stress, the rapidly activated protein kinases SnRK2.4 and SnRK2.6 can phosphorylate SIZ1, which enhances the stability of SIZ1, and participates in the response to osmotic stress. However, the instability of SIZ1S820A protein was independent of COP1, indicating that there might be a new degradation mechanism for SIZ1. Our studies suggest that SnRK2s induced phosphorylation regulates SIZ1 stability to precisely modulates SUMOylation dynamics upon osmotic stresses. Additionally, such a reciprocal regulation by SnRK2 and COP1 might be a general regulation machinery that controls the stability of dozens of proteins.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Tian Sang

PROVIDER: PXD040237 | JPOST Repository | Tue Feb 20 00:00:00 GMT 2024

REPOSITORIES: jPOST

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Project description:Pathak2013 - MAPK activation in response to various biotic stresses MAPK activation mechanism in response to various biotic (fungal and bacterial pathogens) stress conditions in plants This model is described in the article: Modeling of the MAPK machinery activation in response to various abiotic and biotic stresses in plants by a system biology approach. Pathak RK, Taj G, Pandey D, Arora S, Kumar A. Bioinformation 2013; 9(9): 443-449 Abstract: Mitogen-Activated Protein Kinases (MAPKs) cascade plays an important role in regulating plant growth and development, generating cellular responses to the extracellular stimuli. MAPKs cascade mainly consist of three sub-families i.e. mitogen-activated protein kinase kinase kinase (MAPKKK), mitogen-activated protein kinase kinase (MAPKK) and mitogen activated protein kinase (MAPK), several cascades of which are activated by various abiotic and biotic stresses. In this work we have modeled the holistic molecular mechanisms essential to MAPKs activation in response to several abiotic and biotic stresses through a system biology approach and performed its simulation studies. As extent of abiotic and biotic stresses goes on increasing, the process of cell division, cell growth and cell differentiation slow down in time dependent manner. The models developed depict the combinatorial and multicomponent signaling triggered in response to several abiotic and biotic factors. These models can be used to predict behavior of cells in event of various stresses depending on their time and exposure through activation of complex signaling cascades. This model is hosted on BioModels Database and identified by: BIOMD0000000492 . To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models . To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Project description:Pathak2013 - MAPK activation in response to various abiotic stresses MAPK activation mechanism in response to various abiotic stress conditions, such as cold, salt, drought, H2O2, heavy metal and ethylene, in plants This model is described in the article: Modeling of the MAPK machinery activation in response to various abiotic and biotic stresses in plants by a system biology approach. Pathak RK, Taj G, Pandey D, Arora S, Kumar A. Bioinformation 2013; 9(9): 443-449 Abstract: Mitogen-Activated Protein Kinases (MAPKs) cascade plays an important role in regulating plant growth and development, generating cellular responses to the extracellular stimuli. MAPKs cascade mainly consist of three sub-families i.e. mitogen-activated protein kinase kinase kinase (MAPKKK), mitogen-activated protein kinase kinase (MAPKK) and mitogen activated protein kinase (MAPK), several cascades of which are activated by various abiotic and biotic stresses. In this work we have modeled the holistic molecular mechanisms essential to MAPKs activation in response to several abiotic and biotic stresses through a system biology approach and performed its simulation studies. As extent of abiotic and biotic stresses goes on increasing, the process of cell division, cell growth and cell differentiation slow down in time dependent manner. The models developed depict the combinatorial and multicomponent signaling triggered in response to several abiotic and biotic factors. These models can be used to predict behavior of cells in event of various stresses depending on their time and exposure through activation of complex signaling cascades. This model is hosted on BioModels Database and identified by: BIOMD0000000491 . To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models . To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

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	20180415_PHOS_180.raw	Raw
	20180415_PHOS_180.xlsx	Xlsx
	W-C-1.raw	Raw
	W-C-2.raw	Raw
	W-C-3.raw	Raw

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Direct phosphorylation of SIZ1 by SnRK2s kinase to response the osmotic stress in Arabidopsis

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