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Immune Isoform Atlas: Landscape of alternative splicing in human immune cells

ABSTRACT: This dataset contains the global and terminal proteome of THP-1 and LCL cell lines untreated or treated with phorbol 12-myristate 13-acetate (PMA) and interferon-α2 (IFN-α2), respectively. Extracted proteins were digested with trypsin or V8 protease and protein terminal peptides were isolated with strong cation exchange chromatography or metal oxide-based ligand-exchange chromatography. All peptides were labeled with TMT-tag, and a total of 16 channels were used with quadruplicates of untreated LCL, IFN-α2-treated LCL, untreated THP-1, and PMA-treated THP-1 peptides, respectively. The labeled peptides were fractionated into 24 vials by reversed-phase HPLC at high pH conditions.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yasushi Ishihama

PROVIDER: PXD040962 | JPOST Repository | Mon Apr 08 00:00:00 BST 2024

REPOSITORIES: jPOST

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			Action	DRS
	220321HN_THP1NATMTproTrypticWholeFr01.raw	Raw
	220321HN_THP1NATMTproTrypticWholeFr02.raw	Raw
	220321HN_THP1NATMTproTrypticWholeFr03.raw	Raw
	220321HN_THP1NATMTproTrypticWholeFr04.raw	Raw
	220321HN_THP1NATMTproTrypticWholeFr05.raw	Raw

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