Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Phosphoproteome analysis of glioma

ABSTRACT: To identify the key functions of CYLD in glioma, we compared phosphorylation state between WT and CYLD KD in U251 cells.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hirofumi Jono

PROVIDER: PXD043537 | JPOST Repository | Fri Jul 28 00:00:00 BST 2023

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Phospho_siCYLD_U251_IDA.wiff	Wiff
	Phospho_siCYLD_U251_IDA.wiff.scan	Wiff
	Phospho_siN_U251_IDA.wiff	Wiff
	Phospho_siN_U251_IDA.wiff.scan	Wiff
	U251_siCYLD1.wiff	Wiff

Items per page:

1 - 5 of 20

Similar Datasets

E-GEOD-47777

Project description:Whole genome expression data comparison between WT and Cebpg-/- MEFs.

2015-07-17 | E-GEOD-47777 | ExpressionAtlas

Control of developmentally primed erythroid genes by combinatorial corepressor actions

Project description:How transcription factors (TFs) cooperate within large protein complexes to allow rapid modulation of gene expression during development is still largely unknown. Here we show that the key haematopoietic LIM-domain-binding protein-1 (LDB1) TF complex contains several activator and repressor components that together maintain an erythroid-specific gene expression programme primed for rapid activation until differentiation is induced. A combination of proteomics, functional genomics and in vivo studies presented here identifies known and novel co-repressors, most notably the ETO2 and IRF2BP2 proteins, involved in maintaining this primed state. The ETO2â??IRF2BP2 axis, interacting with the NCOR1/SMRT co-repressor complex, suppresses the expression of the vast majority of archetypical erythroid genes and pathways until its decommissioning at the onset of terminal erythroid differentiation. Our experiments demonstrate that multimeric regulatory complexes feature a dynamic interplay between activating and repressing components that determines lineage-specific gene expression and cellular differentiation. ChIP-Sequencing profiles of the IRF2BP2, GFI1B and LSD1 proteins were generated using mouse erythroleukemia (MEL) cells. RNA-seq experiments of Irf2bp2-WT, Irf2bp2-KD, Eto2-WT, Eto2-KD, Gfi1b-WT, Gfi1b-KD, Lsd1-WT, Lsd1-KD, MEL-non-induced, and MEL-induced stages were performed using standard RNA-seq protocol. Illumina HiSeq 2000 (standard TruSeq RNA sequencing protocol) was used for the sequencing.

2015-11-19 | E-GEOD-59859 | biostudies-arrayexpress

The pancreatic cancer stem cell marker CD44v6 affects transcription, translation and signaling: consequences on exosome composition and delivery II

Project description:The miRNA profile between different pancreatic adenocarcinoma cells (A818.4, Capan-1) and different colorectal carcinoma cells (SW948, HT-29). The impact of a knockdown (kd) of function-relevant cancer stem cell markers (CD44v6, Tspan8, CD151, claudin7) on the miRNA profile. The kd cell miRNA profiles were compared with the wt cell as well as between the different kd miRNA profiles.

2018-08-25 | GSE119032 | GEO

E-GEOD-38283

Project description:Gene expression profile between glioma associated macrophages and normal brain tissue associated macrophages

2015-09-30 | E-GEOD-38283 | ExpressionAtlas

Phosphoproteome of Monascus purpureus

Project description:MpSTE1 mediates the phosphoylation of downstream proteins which control hyphal branching in M. purpureus. The phosphoproteomes of the wild-type (WT) and ΔmpSTE1 strains were compared to pinpoint proteins specifically phosphorylated in an MpSTE1-dependent manner. Since the gene mpSTE1 was disrupted in the mutant, its targets should not undergo phosphorylation compared to the WT strain. The data were filtered for phosphorylated peptides present in all WT replicates but absent in all ΔmpSTE1 replicates.

2023-12-29 | PXD048208 |

E-GEOD-60823

Project description:Expression data from +Pi and -Pi treeatment between WT and spx1spx2 double mutant

2015-05-14 | E-GEOD-60823 | ExpressionAtlas

The pancreatic cancer stem cell marker CD44v6 affects transcription, translation and signaling: consequences on exosome composition and delivery I

2018-08-25 | GSE119031 | GEO

Expression data from thymic lymphomas of Lck-MyrAkt positive mice with WT or KD mTOR expression

Project description:Lck-MyrAkt2 mice develop spontaneous thymic lymphomas at approximately 100-200 days of age, driven in part by a consitutatively-active AKT (due to myristoylation). mTOR Knock Down mice were crossed with Lck-MyrAkt postive mice to model the affects of decreasing mTOR activity on tumors with an activated PI3K/AKT/MTOR pathway. Lck-Akt/mTOR KD mice had prolonged survival compared to the Lck-Akt/mTOR WT mice. We used microarrays to compare the transcriptome in thymic lymphomas between Lck-Akt positive, mTOR WT and Lck-Akt positive, mTOR KD mice. Four thymic lymphomas from Lck-Akt/mTOR WT mice were compared to three thymic lymphomas from Lck-Akt/mTOR KD mice.

2015-09-15 | E-MTAB-3242 | biostudies-arrayexpress

Gene expression analysis of kidney primary tubular epithelial cells (PTECs), SETD2-KD PTECs, PBRM1-KD PTECs and ccRCC derived cell lines

Project description:Transcriptional profiling of human WT-PTECs, SETD2-KD PTECs, PBRM1-KD PTECs, and 10 different ccRCC derived cell lines. ccRCC derived cell lines showed distict expression signatures as compared to PTECs, some of them also present in SETD2-KD PTECs, and/or PBRM1-KD PTECs.

2017-11-01 | GSE89214 | GEO

E-GEOD-28227

Project description:Comparison of gene expression profiles between erf5/6 and WT Arabidopsis in response to chitooctaose

2015-05-22 | E-GEOD-28227 | ExpressionAtlas