Proteomics

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Phosphoproteomic analysis of mpk1/2/7/14 treated with mannitol or abscisic acid


ABSTRACT: Abscisic acid (ABA) is a phytohormone that promotes leaf senescence in response to environmental stress. We previously identified methyl CpG-binding domain 10 (MBD10) as a phosphoprotein that becomes differentially phosphorylated after ABA treatment in Arabidopsis. ABA-induced leaf senescence was delayed in mbd10 knockout plants but accelerated in MBD10-overexpressing plants, suggesting that MBD10 positively regulates ABA-induced leaf senescence. ABA-induced phosphorylation of MBD10 occurs in planta on Thr-89, and our results demonstrated that Thr-89 phosphorylation is essential for MBD10’s function in leaf senescence. The in vivo phosphorylation of Thr-89 in MBD10 was significantly downregulated in a quadruple mutant of group C MAPKs (mpk1/2/7/14), and group C MAPKs directly phosphorylated MBD10 in vitro.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Taishi Umezawa 

PROVIDER: PXD045815 | JPOST Repository | Sat Sep 30 00:00:00 BST 2023

REPOSITORIES: jPOST

Dataset's files

Source:
Action DRS
mpkQKO_Phosphoproteome_220125.msf Msf
mpkQKO_Phosphoproteome_220125.pdResult Other
test220122_WT_ABA01.raw Raw
test220122_WT_ABA02.raw Raw
test220122_WT_ABA03.raw Raw
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