Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Elongator Complex Elp1-6

ABSTRACT: Human elongator complex Elp1-6 purified from insect cells was analysed by crosslinking mass spectrometry.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Juri Rappsilber

PROVIDER: PXD045927 | JPOST Repository | Thu May 16 00:00:00 BST 2024

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	B220917_02_Lumos_AR_CO_120_elon_SDA019038_SEC7_rep1.raw	Raw
	B220917_03_Lumos_AR_CO_120_elon_SDA019038_SEC7_rep2.raw	Raw
	B220917_04_Lumos_AR_CO_120_elon_SDA019038_SEC8_rep1.raw	Raw
	B220917_05_Lumos_AR_CO_120_elon_SDA019038_SEC8_rep2.raw	Raw
	B220917_06_Lumos_AR_CO_120_elon_SDA019038_SEC9_rep1.raw	Raw

Items per page:

1 - 5 of 20

Similar Datasets

Mouse Elongator Complex Elp1-6

Project description:Mouse elongator complex Elp1-6 purified from insect cells was analysed by crosslinking mass spectrometry.

2024-05-16 | PXD045928 | JPOST Repository

Crosslinking mass spectrometry analysis of the Trypanosoma brucei KKT23 complex

Project description:KKT23 is a kinetoplastid kinetochore protein that has structural similarity to the GCN5 acetyltransferase domain. We performed crosslinking mass spectrometry of the recombinant KKT3-KKT22-KKT23 complex purified from Sf9 insect cells.

2024-08-19 | PXD052923 | Pride

Yeast Elongator Complex Elp1-6

Project description:Elongator complex Elp1-6 purified from Saccharomyces cerevisiae was analysed by crosslinking mass spectrometry.

2022-11-24 | PXD038060 | JPOST Repository

Identification of recombinant settlement-inducing protein complex from Amphibalanus amphitrite by LC-MS/MS

Project description:Settlement-inducing protein complex (SIPC) is a protein that acts as a phoromone cue to attract conspecific of the barnacle Amphibalanus amphitrite to settle on a substrate. Recombinant settlement-inducing protein complex was expressed in insect Sf9 cells through a baculovirus overexpression system, purified and analysed by mass spectrometry to confirm its identity.

2019-01-07 | PXD006858 | Pride

Mass spectrometry analysis of chromosomal passenger complex proteins and kinetochore proteins in Trypanosoma brucei

Project description:We performed chemical crosslinking mass spectrometry of native protein complexes purified from Trypanosoma brucei procyclic cells to obtain protein-protein interaction information for the chromosomal passenger complex (CPC) and kinetochore complex. Crosslinks among CPC subunits were detected using YFP-AUK1, while those among KKT8 complex subunits were detected in the YFP-KKIP1 sample. We also performed immunoprecipitation and mass spectrometry of CPC subunits (full-length proteins and fragments) to identify co-purifying proteins.

2023-10-13 | PXD045987 | Pride

BBSome Core Complex

Project description:Chemical crosslinking coupled with mass spectrometry data for the BBSome Core Complex

2017-08-22 | MSV000081472 | MassIVE

GNPS - proteomics and lipidomics of LAT1-4F2hc complex

Project description:proteomics and lipidomics analysis of recombinant LAT1-4F2hc complex purified from HEK293S cells

2024-04-06 | MSV000094478 | GNPS

α-Synuclein promotes H4R3me2s through interacting with BAF-PRMT5 complex

Project description:We conducted a screening for nuclear proteins which interact with α-synuclein by mass-spectrometry. As a result, we found that α-synuclein interact with BAF complex. Furthermore, overexpressed α-synuclein increased global H4R3me2s by promoting interaction between BAF complex and PRMT5 in cultered cells. In this study, we explored the influence of overexpressed α-synuclein on H4R3me2s by ChIP-seq using anti-H4R3me2s antibody.

2023-11-23 | GSE228331 | GEO

Crosslinking MS to elucidate the Architecture of the Nucleosome Remodelling and Deacetylase (NuRD) complex

Project description:The Nucleosome Remodeling and Deacetylase (NuRD) complex is essential for development in complex animals but has been refractory to detailed analysis because of its low abundance and resistance to recombinant production. In combination with other techniques, crosslinking mass spectrometry was used to elucidate the structure of the Nucleosome Remodelling and Deacetylase (NuRD) complex. Natively-purified NuRD, and four recombinantly-expressed NuRD subcomplexes, and a PWWP2A-MTA-HDAC-RBBP alternative ‘NuRD-like’ complex were subjected to crosslinking with DSS, ADH, BS3 and DMTMM.

2021-01-08 | PXD010111 | Pride

Identifying insect cell proteins copurified with wild-type human UBR4/KCMF1/CALM1 complex and different domain deletions

Project description:The UBR4/KCMF1/CALM1 complex is an important general protein quality control enzyme which extends ubiquitin chains on pre-ubiquitinated proteins to target them for degradation. We observed by cryogenic electron microscopy that the complex purified recombinantly from insect cells is bound to a potential substrate which cannot be identified by experimental density. However, we could determine that the copurified protein is bound to a certain domain in the complex termed the ZZ-DZB domain. To identify the copurified insect cell proteins we performed LC-MS analysis of the purified wild-type complex and a complex with the ZZ-DZB domain deleted. We compared the two datasets to identify proteins which were highly enriched in the wildtype dataset but lost in the ZZ-DZB deletion dataset. To gain additional insight into the role of the domains of UBR4 in substrate recognition, we repeated this approach with two other domain deletions – the BS2 domain and the UBL domain. As the UBR4/KCMF1/CALM1 complex is expected to recognize proteins by the processing of their N-termini, we also performed digests with three different proteases to try and identify the native N-termini of the co-purified proteins.

2025-09-02 | PXD063485 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data