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Proteomic analysis of cochlear perilymph in a mouse model of Alzheimer's disease

ABSTRACT: This study referred to the changes in protein abundance of App(NL-GF/NL-GF) and App(wt/wt) mice perilymph. Liquid chromatography-tandem mass spectrometry with MS2-based quantification of tandem mass tag was utilized.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Hiroshi Hibino

PROVIDER: PXD046317 | JPOST Repository | Mon Apr 15 00:00:00 BST 2024

REPOSITORIES: jPOST

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Dataset's files

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			Action	DRS
	20220915_PLF_Mouse_WT_TMT_3h.mgf	Mgf
	20220915_PLF_Mouse_WT_TMT_3h.mzTab	Mztab
	20220915_PLF_Mouse_WT_TMT_3h.raw	Raw

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Publications

Disturbance in the protein landscape of cochlear perilymph in an Alzheimer's disease mouse model.

Fukuda Masatoshi M Okanishi Hiroki H Ino Daisuke D Ono Kazuya K Kawamura Satoru S Wakai Eri E Miyoshi Tsuyoshi T Sato Takashi T Ohta Yumi Y Saito Takashi T Saido Takaomi C TC Inohara Hidenori H Kanai Yoshikatsu Y Hibino Hiroshi H

PloS one 20240510 5

Hearing loss is a pivotal risk factor for dementia. It has recently emerged that a disruption in the intercommunication between the cochlea and brain is a key process in the initiation and progression of this disease. However, whether the cochlear properties can be influenced by pathological signals associated with dementia remains unclear. In this study, using a mouse model of Alzheimer's disease (AD), we investigated the impacts of the AD-like amyloid β (Aβ) pathology in the brain on the cochl ...[more]

PMID: 38728348

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Project description:To investigate the effects of diabetic conditions on the development of AD pathology, we fed wild-type and Alzheimer's disease model (App[NL-F/NL-F]) mice with control or high fat diet from 6 months after birth for 12 months, then examined behavior, brain pathology and gene expression profiles. Both groups of mice fed with high fat diet exhibited diabetic conditions, and App[NL-F/NL-F] mice exhibited aggravated cognitive impairment with enhanced Alzheimer's disease pathology.

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Comparative profiling of hippocampal gene expression in AppNL-G-F knock-in mouse model of Alzheimer's disease.

Project description:To identify molecular pathological alterations in Alzheimer’s disease (AD) brains, we had previously performed comparative gene expression profiling with human gene array using RNAs prepared from postmortem human brains donated for the Hisayama study. The hippocampi from AD brains showed the most significant alteration in gene expression profile (PMID: 23595620). In the present study, in order to obtain a comprehensive view of gene expression profiles in AD hippocampus, we applied human transcriptome array and deep RNA-sequencing to the same samples and reanalyzed the gene array data, and performed integrative analysis of all 3 methods. We also applied gene array to hippocampus of 6-month-old App[NL-G-F/NL-G-F] knock-in AD model mice, and at last we performed the interspecies comparison. IPA analysis of the top significantly altered genes derived from integrative analysis of all 3 methods in human AD hippocampus revealed that neurotransmission, synaptic transmission and development of neurons are the top significantly decreased functions. In hippocampi of App[NL-G-F/NL-G-F] mice which exhibit significant amyloidosis but not neuronal degeneration, we found that expression of Gfap (astrocyte marker) and Cd68 (microglia marker) genes are significantly upregulated together with genes categorized in the immune response of cells. Comparing the human and mouse data, we found major common genes are related to recruitment of inflammatory cells. Amyloid-beta accumulation is the major hallmark of AD at 6 month-old App[NL-G-F/NL-G-F] mice, therefore our data suggests that amyloid-beta accumulation increases the recruitment of inflammatory cells in early stage of AD brain, prior to neuronal degeneration.

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