Dataset Information

HaCaT cells exposed to blue and red light

ABSTRACT: HaCaT cells exposed to blue or red light for 3 days 12:12 L:D cycle

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Massimo Bracci

PROVIDER: PXD046776 | JPOST Repository | Tue Nov 28 00:00:00 GMT 2023

REPOSITORIES: jPOST

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Publications

Keratinocytes Exposed to Blue or Red Light: Proteomic Characterization Showed Cytoplasmic Thioredoxin Reductase 1 and Aldo-Keto Reductase Family 1 Member C3 Triggered Expression.

Lazzarini Raffaella R Tartaglione Maria Fiorella MF Ciarapica Veronica V Piva Francesco F Giulietti Matteo M Fulgenzi Gianluca G Martelli Margherita M Ledda Caterina C Vitale Ermanno E Malavolta Marco M Santarelli Lory L Bracci Massimo M

International journal of molecular sciences 20231110 22

Several cell-signaling mechanisms are activated by visible light radiation in human keratinocytes, but the key regulatory proteins involved in this specific cellular response have not yet been identified. Human keratinocytes (HaCaT cells) were exposed to blue or red light at low or high irradiance for 3 days in cycles of 12 h of light and 12 h of dark. The cell viability, apoptotic rate and cell cycle progression were analyzed in all experimental conditions. The proteomic profile, oxidative stre ...[more]

PMID: 38003379

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Project description:The cold acclimation process is regulated by many factors like ambient temperature, day length, light intensity, or hormonal status. Experiments with plants grown under different light-quality conditions indicate that the plant response to cold is also a light-quality-dependent process. Here, the role of light quality in the cold response was studied in one-month-old Arabidopsis thaliana (Col‐0) plants exposed for one week to 4 °C at short‐day conditions under white (100 and 20 μmol m‐2s‐1), blue or red (20 μmol m‐2s‐1) light conditions. An upregulated expression of CBF1, an inhibition of photosynthesis, and an increase in membrane damage showed that blue light enhanced the effect of low temperature. Interestingly, cold-treated plants under blue and red light showed only limited freezing tolerance compared to white light cold-treated plants. Next, the specificity of the light quality signal in cold response was evaluated in Arabidopsis accessions originating from different and contrasting latitudes. In all but one Arabidopsis accessions, blue light increased the effect of cold on photosynthetic parameters and electrolyte leakage. This effect was not found for Ws-0, which lacks functional CRY2 protein, indicating its role in the cold response. Proteomics data confirmed significant differences between red and blue light treated plants at low temperature and showed that the cold response is highly accession specific. In general, blue light increased mainly the cold-stress related proteins and red light induced higher expression of chloroplast-related proteins, which correlated with higher photosynthetic parameters in red light cold-treated plants. Altogether, our data suggest that light modulates two distinct mechanisms during the cold treatment - red light driven cell function maintaining program and blue light activated specific cold response. The importance of mutual complementarity of these mechanisms was demonstrated by significantly higher freezing tolerance of cold-treated plants under white light.

			Action	DRS
	HaCaT%20BLU1.raw	Raw
	HaCaT%20BLU2.raw	Raw
	HaCaT%20BLU3.raw	Raw
	HaCaT%20BLU4.raw	Raw
	HaCaT%20CTRL1.raw	Raw