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Cargo analysis of autophagic bodies in Saccharomyces cerevisiae

ABSTRACT: Isolated yeast autophagic bodies and the total cell lysate were analyzed by mass spectrometry.

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Eigo Takeda

PROVIDER: PXD052405 | JPOST Repository | Thu Aug 22 00:00:00 BST 2024

REPOSITORIES: jPOST

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			Action	DRS
	01_R2_TL_WT_1.raw	Raw
	01_R2_TL_WT_2.raw	Raw
	01_R2_TL_WT_3.raw	Raw
	02_N2_TL_WT_1.raw	Raw
	02_N2_TL_WT_2.raw	Raw

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Publications

Receptor-mediated cargo hitchhiking on bulk autophagy.

Takeda Eigo E Isoda Takahiro T Hosokawa Sachiko S Oikawa Yu Y Hotta-Ren Shukun S May Alexander I AI Ohsumi Yoshinori Y

The EMBO journal 20240516 15

While the molecular mechanism of autophagy is well studied, the cargoes delivered by autophagy remain incompletely characterized. To examine the selectivity of autophagy cargo, we conducted proteomics on isolated yeast autophagic bodies, which are intermediate structures in the autophagy process. We identify a protein, Hab1, that is highly preferentially delivered to vacuoles. The N-terminal 42 amino acid region of Hab1 contains an amphipathic helix and an Atg8-family interacting motif, both of ...[more]

PMID: 38755257

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