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Reanalysis dataset of JPST000208

ABSTRACT: Quantitative proteome analyses of colorectal cancer cell lines (metastatis: SW620, non-metastatis: SW480) were carried out using SCX-RP-MS/MS with SILAC labeling(SW480: light-label, SW620: heavy-label). For this reanalysis, MS Datasets were processed for peak picking with MaxQuant v1.6.2.10, and the generated peak lists were searched against human entries of UniProt/Swiss-Prot release 2018_10 using three search engines: MaxQuant v1.6.2.10, X!Tandem v2015.04.01.1, and Comet 2015.02 rev. 0. The decoy database was generated by reversing sequences from the database. Then, the sum of the total number of matched b/y ions and the total number of amino acids comprising the peptide sequence tag were counted to score the reliability of peptide identification independent of these search engines. Finally, the 1% false discovery rate was controlled by the target-decoy approach with this score.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yasushi Ishihama

PROVIDER: RPXD018481 | JPOST Repository | Wed May 27 00:00:00 BST 2020

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	111222_HL01.RAW	Raw
	111222_HL02.RAW	Raw
	111222_HL03.RAW	Raw
	111222_HL04.RAW	Raw
	111222_LH10.RAW	Raw

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