MEMA HCC1954 Staining Set 100 (Lapatinib treatment)
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ABSTRACT: HCC1954 cells were grown in media with Lapatinib in 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(Basal lineage), KRT19 (luminal lineage) and EdU (S phase activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.
PROVIDER: LDS-1477 | LINCS | 2016-11-10
REPOSITORIES: LINCS
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