Dataset Information

Alpha-lytic proteases

ABSTRACT: S. pombe proteome digested separately by four proteases and analyzed with an LTQ-Orbitrap Velos with a data-dependent decision tree method.

PROVIDER: MSV000078516 | MassIVE | Mon Dec 23 16:44:00 GMT 2013

REPOSITORIES: MassIVE

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Publications

Expanding proteome coverage with orthogonal-specificity α-lytic proteases.

Meyer Jesse G JG Kim Sangtae S Maltby David A DA Ghassemian Majid M Bandeira Nuno N Komives Elizabeth A EA

Molecular & cellular proteomics : MCP 20140114 3

Bottom-up proteomics studies traditionally involve proteome digestion with a single protease, trypsin. However, trypsin alone does not generate peptides that encompass the entire proteome. Alternative proteases have been explored, but most have specificity for charged amino acid side chains. Therefore, additional proteases that improve proteome coverage through cleavage at sequences complementary to trypsin's may increase proteome coverage. We demonstrate the novel application of two proteases f ...[more]

PMID: 24425750

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