Proteomics

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LC-MS/MS analyses of purified virions of influenza A and B viruses, grown in a range of hosts.


ABSTRACT: LC-MS/MS analyses of purified virions of influenza A and B viruses, grown in a range of hosts. Described in Hutchinson et al. 'Conserved and host-specific features of influenza virion architecture.'

INSTRUMENT(S): LTQ Orbitrap, Q Exactive

ORGANISM(S): Bos Taurus (ncbitaxon:9913) Canis Familiaris (canine) Influenza Viruses Gallus Gallus (ncbitaxon:9031)

SUBMITTER: Edward Hutchinson; Ervin Fodor 

PROVIDER: MSV000078740 | MassIVE | Mon Jun 16 06:33:00 BST 2014

REPOSITORIES: MassIVE

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Conserved and host-specific features of influenza virion architecture.

Hutchinson Edward C EC   Charles Philip D PD   Hester Svenja S SS   Thomas Benjamin B   Trudgian David D   Martínez-Alonso Mónica M   Fodor Ervin E  

Nature communications 20140916


Viruses use virions to spread between hosts, and virion composition is therefore the primary determinant of viral transmissibility and immunogenicity. However, the virions of many viruses are complex and pleomorphic, making them difficult to analyse in detail. Here we address this by identifying and quantifying virion proteins with mass spectrometry, producing a complete and quantified model of the hundreds of host-encoded and viral proteins that make up the pleomorphic virions of influenza viru  ...[more]

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