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DdDT results from 2014 MCP alpha-lytic protease proteomics paper

ABSTRACT: ddDT runs of peptides from four separate protease digestions. CID for z=2, ETD for z>=3

INSTRUMENT(S): LTQ Orbitrap Velos ETD

ORGANISM(S): Schizosaccharomyces Pombe (ncbitaxon:4896)

SUBMITTER: EA Komives

PROVIDER: MSV000079025 | MassIVE | Fri Jan 30 12:35:00 GMT 2015

SECONDARY ACCESSION(S): PXD001771

REPOSITORIES: MassIVE

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Publications

Expanding proteome coverage with orthogonal-specificity α-lytic proteases.

Meyer Jesse G JG Kim Sangtae S Maltby David A DA Ghassemian Majid M Bandeira Nuno N Komives Elizabeth A EA

Molecular & cellular proteomics : MCP 20140114 3

Bottom-up proteomics studies traditionally involve proteome digestion with a single protease, trypsin. However, trypsin alone does not generate peptides that encompass the entire proteome. Alternative proteases have been explored, but most have specificity for charged amino acid side chains. Therefore, additional proteases that improve proteome coverage through cleavage at sequences complementary to trypsin's may increase proteome coverage. We demonstrate the novel application of two proteases f ...[more]

PMID: 24425750

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