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Proteome analysis of human lymphotropic tumor viruses

ABSTRACT: Detection/identification of virola proteins from tumor cells

OTHER RELATED OMICS DATASETS IN: GSE34538PRJNA151379

INSTRUMENT(S): LTQ XL ETD, LTQ

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Richard D. Smith

PROVIDER: MSV000079036 | MassIVE | Thu Feb 12 19:26:00 GMT 2015

SECONDARY ACCESSION(S): PXD001811

REPOSITORIES: MassIVE

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Coupled transcriptome and proteome analysis of human lymphotropic tumor viruses: insights on the detection and discovery of viral genes.

Dresang Lindsay R LR Teuton Jeremy R JR Feng Huichen H Jacobs Jon M JM Camp David G DG Purvine Samuel O SO Gritsenko Marina A MA Li Zhihua Z Smith Richard D RD Sugden Bill B Moore Patrick S PS Chang Yuan Y

BMC genomics 20111220

<h4>Background</h4>Kaposi's sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV) are related human tumor viruses that cause primary effusion lymphomas (PEL) and Burkitt's lymphomas (BL), respectively. Viral genes expressed in naturally-infected cancer cells contribute to disease pathogenesis; knowing which viral genes are expressed is critical in understanding how these viruses cause cancer. To evaluate the expression of viral genes, we used high-resolution separation and mass spec ...[more]

PMID: 22185355

Similar Datasets

Coupled Transcriptome and Proteome Analysis of Human Lymphotropic Tumor Viruses: Insights on the Detection and Discovery of Viral Genes

Project description:Kaposi’s sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV) are related human tumor viruses that cause primary effusion lymphomas (PEL) and Burkitt’s lymphomas (BL), respectively. Viral genes expressed in naturally-infected cancer cells contribute to disease pathogenesis; knowing which viral genes are expressed is critical in understanding how these viruses cause cancer. To evaluate the expression of viral genes, we used high-resolution separation and mass spectrometry coupled with custom tiling arrays to align the viral proteomes and transcriptomes of three PEL and two BL cell lines under latent and lytic culture conditions. The majority of viral genes were efficiently detected at the transcript and/or protein level on manipulating the viral life cycle. Overall the correlation of expressed viral proteins and transcripts was highly complementary in both validating and providing orthogonal data with latent/lytic viral gene expression. Our approach also identified novel viral genes in both KSHV and EBV, and extends viral genome annotation. The expression of several previously uncharacterized latent genes were validated at both transcript and protein levels. This systems biology approach coupling proteome and transcriptome measurements provides a comprehensive view of viral gene expression that could not have been attained using each methodology independently. Detection of viral proteins in combination with viral transcripts is a potentially powerful method for establishing virus-disease relationships.

2011-12-30 | GSE34538 | GEO

Coupled Transcriptome and Proteome Analysis of Human Lymphotropic Tumor Viruses: Insights on the Detection and Discovery of Viral Genes

Project description:Coupled Transcriptome and Proteome Analysis of Human Lymphotropic Tumor Viruses: Insights on the Detection and Discovery of Viral Genes

| PRJNA151379 | ENA

Coupled transcriptome and proteome analysis of human lymphotropic tumor viruses: insights on the detection and discovery of viral genes.

Project description:BackgroundKaposi's sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV) are related human tumor viruses that cause primary effusion lymphomas (PEL) and Burkitt's lymphomas (BL), respectively. Viral genes expressed in naturally-infected cancer cells contribute to disease pathogenesis; knowing which viral genes are expressed is critical in understanding how these viruses cause cancer. To evaluate the expression of viral genes, we used high-resolution separation and mass spectrometry coupled with custom tiling arrays to align the viral proteomes and transcriptomes of three PEL and two BL cell lines under latent and lytic culture conditions.ResultsThe majority of viral genes were efficiently detected at the transcript and/or protein level on manipulating the viral life cycle. Overall the correlation of expressed viral proteins and transcripts was highly complementary in both validating and providing orthogonal data with latent/lytic viral gene expression. Our approach also identified novel viral genes in both KSHV and EBV, and extends viral genome annotation. Several previously uncharacterized genes were validated at both transcript and protein levels.ConclusionsThis systems biology approach coupling proteome and transcriptome measurements provides a comprehensive view of viral gene expression that could not have been attained using each methodology independently. Detection of viral proteins in combination with viral transcripts is a potentially powerful method for establishing virus-disease relationships.

| S-EPMC3282826 | biostudies-literature

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Project description:A CHG microarray for detection and identification of bacteria and viruses pathogenic on maize

2016-07-01 | GSE69895 | GEO

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19 K14Cre; p53F/F mouse mammary tumor analysis

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2010-05-26 | E-GEOD-7794 | biostudies-arrayexpress

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MaizePath: A CHG microarray for detection and identification of bacteria and viruses pathogenic on maize

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