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Molecular Architecture of the Yeast Mediator Complex

ABSTRACT: Cross-linking MS data from the yeast Mediator complex. Cross-linking was performed using either BS3 or 1:1 mix of d0:d12 DSS. Includes unfractionated, SEC enriched, high pH reverse phase fractionated, DDA and inclusion list generated files.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Roger D. Kornberg

PROVIDER: MSV000079237 | MassIVE | Thu Aug 13 15:44:00 BST 2015

SECONDARY ACCESSION(S): PXD002723

REPOSITORIES: MassIVE

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Molecular architecture of the yeast Mediator complex.

Robinson Philip J PJ Trnka Michael J MJ Pellarin Riccardo R Greenberg Charles H CH Bushnell David A DA Davis Ralph R Burlingame Alma L AL Sali Andrej A Kornberg Roger D RD

eLife 20150924

The 21-subunit Mediator complex transduces regulatory information from enhancers to promoters, and performs an essential role in the initiation of transcription in all eukaryotes. Structural information on two-thirds of the complex has been limited to coarse subunit mapping onto 2-D images from electron micrographs. We have performed chemical cross-linking and mass spectrometry, and combined the results with information from X-ray crystallography, homology modeling, and cryo-electron microscopy ...[more]

PMID: 26402457

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