Differential stoichiometry among core ribosomal proteins

ABSTRACT: Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its dysregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testing such variability requires direct and precise quantification of RPs. We used mass-spectrometry to directly quantify RPs across monosomes and polysomes of mouse embryonic stem cells (ESC) and budding yeast. Our data show that the stoichiometry among core RPs in wild-type yeast cells and ESC depends both on the growth conditions and on the number of ribosomes bound per mRNA. Furthermore, we find that the fitness of cells with a deleted RP-gene is inversely proportional to the enrichment of the corresponding RP in polysomes. Together, our findings support the existence of ribosomes with distinct protein composition and physiological function.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Saccharomycetales (ncbitaxon:4892) Mus Musculus (ncbitaxon:10090)

SUBMITTER: Nikolai Slavov 

PROVIDER: MSV000079280 | MassIVE | Fri Aug 28 12:21:00 BST 2015

SECONDARY ACCESSION(S): PXD002816

REPOSITORIES: MassIVE