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Embryo_proteome

ABSTRACT: We collected over 8000 mouse embryos of each developmental stage (zygote, 2-cell, 4-cell, 8-cell, morula, blastocyst) and performed tandem mass tag (TMT)-based quantitative mass spectrometry and identified nearly 5000 proteins for each stage. In-depth analysis indicates that protein expression profiles of zygote, morula and blastocyst show apparent difference from 2- to 8-cell embryos due to the maternal-totipotent-differentiation transition. We further identified novel factors and proved that they play important roles in determining pre-implantation embryo development

OTHER RELATED OMICS DATASETS IN: PRJNA417454

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Lujian Liao, Shaorong Gao

PROVIDER: MSV000079417 | MassIVE | Thu Dec 10 00:28:00 GMT 2015

SECONDARY ACCESSION(S): PXD003315

REPOSITORIES: MassIVE

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Publications

Protein Expression Landscape of Mouse Embryos during Pre-implantation Development.

Gao Yawei Y Liu Xiaoyu X Tang Bin B Li Chong C Kou Zhaohui Z Li Lin L Liu Wenqiang W Wu You Y Kou Xiaochen X Li Jingyi J Zhao Yanhong Y Yin Jiqing J Wang Hong H Chen She S Liao Lujian L Gao Shaorong S

Cell reports 20171201 13

Pre-implantation embryo development is an intricate and precisely regulated process orchestrated by maternally inherited proteins and newly synthesized proteins following zygotic genome activation. Although genomic and transcriptomic studies have enriched our understanding of the genetic programs underlying this process, the protein expression landscape remains unexplored. Using quantitative mass spectrometry, we identified nearly 5,000 proteins from 8,000 mouse embryos of each stage (zygote, 2- ...[more]

PMID: 29281840

Similar Datasets

Protein expression landscape of mouse embryos during pre-implantation development

Project description:Pre-implantation embryo development is an intricate and precisely regulated process orchestrated by maternally inherited proteins and newly synthesized proteins following zygotic genome activation. Although genomic and transcriptomic studies have enriched our understanding of genetic programs underlying this process, the protein expression landscape remains unexplored. Using quantitative mass spectrometry, we identified nearly 5000 proteins from 8000 mouse embryos of each stage (zygote, 2-cell, 4-cell, 8-cell, morula, blastocyst). We found that protein expression of zygote, morula and blastocyst show apparent difference from 2- to 8-cell embryos. Analysis of protein phosphorylation led to extraction of critical kinases and signal transduction pathways. We identified novel factors and proved that they play important roles in early embryo development. Combined analysis of transcriptomic and proteomic data reveals coordinated control of RNA degradation, transcription and translation, and identifies novel exon junction-derived peptides. Our study provides an invaluable resource for further mechanistic studies and suggests novel players governing pre-implantation embryo development.

2018-01-09 | GSE106612 | GEO

Comparative maternal protein profiling of mouse biparental and uniparental embryos

Project description:During oogenesis, RNAs and proteins from maternal genome will be accumulated and they are the most important regulatory factors in early embryonic development. Parthenogenesis provides a unique source to investigate the influence of maternal genomes in early mammalian development and is proposed as an experimental tool to investigate embryo development which may solve many of the ethical concerns. Using label-free quantitative mass sepctrometry (MS), we systematically monitored protein expression profiles from six stages during pre-implantation development used by parthenogenesis model of mouse: pronucleus-, 2-cell, 4-cell, and 8-cell embryo, morula, and blastocyst. They are labeled as PA, PA-2, PA-4, PA-8, PAMO, PABL, respectively. For each stage, 6,000 embryos were used, and the experiment was performed in three biological replicates, and they had 2,048 proteins in common.

2022-07-15 | PXD029532 |

Human embryo and trophoblast proteome

Project description:Comprehensive quantitative proteomic study of human pre-implantation embryo stages reveal dynamic proteome landscape from M2, 8-cell and blastocyst stage, and during trophoblast stem cell (TS) differentiation. Identified key factors in early human embryos and lineage-specific trophoblast proteome profiles, correlated with transcriptomic analyses. This direct proteomic analysis provides a comprehensive analysis of the dynamic protein expression in human embryos during pre-implantation development and a powerful resource to enable further mechanistic studies on human trophoblast development and function.

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Light induced injury in mouse embryos revealed by single-cell RNA sequencing

Project description:We adopt mouse pre-implantation embryos to evaluate the effects of different exposure time and illumination intensity. The damage-causing time and intensity is chosen to treat embryos at 2-cell stage. Single-cell transcriptomes from 2-cell to blastocyst stage are analyzed under both control and high-intensity light conditions. These data show that embryo cells at each embryonic stage can be categorized into different light conditions. Further analyses of differentially expressed genes and relative GO terms reveal the light-induced injury as well as the potential repair response after high-intensity illumination treatment. Maternal-to-zygote transition is also affected through the failure of removing maternal RNAs and deactivating zygotic genomes. In conclusion, our work revealed integrated response of high-intensity light, involving the long-lasting injury effects, damage repair mechanism and blastocyst morphology changes.

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Reprogramming of H3K9me3-dependent heterochromatin during mammalian early embryo development [WGBS]

Project description:H3K9me3-dependent heterochromatin is considered as one of the major barriers for cell fate changes, and must be reprogrammed during fertilization to reactivate highly specialized paternal and maternal genome to establish totipotency. However, the molecular details are lacked for early embryos due to the limited materials. Here we map the genome-wide distribution of H3K9me3 modification in the early embryo as well as in the cell fate determined embryonic tissues after implantation. We find that H3K9me3 exhibits distinct dynamic features in promoters and retro-transposons. Both maternal and paternal genome undergo large scale of H3K9me3 reestablishment after fertilization, and the imbalance of maternal H3K9me3 signal over paternal last until the blastocyst stage. The rebuilding of H3K9me3 on LTR retro-transposons maintains its repression state after the global DNA demethylation, and we further discover that Chaf1a is essential for the establishment of H3K9me3 on LTRs and the loss function of Chaf1a leads to embryo development failure. Finally, we find that lineage specific H3K9me3 is established after lineage commitment in post-implantation embryos. Thus, our data demonstrate that H3K9me3-dependent heterochromatin undergoes dramatic reprogramming during early embryo development and the establishment of H3K9me3 on LTRs is essential for proper embryo development.

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Reprogramming of H3K9me3-dependent heterochromatin during mammalian early embryo development [RNA-seq]

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Reprogramming of H3K9me3-dependent heterochromatin during mammalian early embryo development [ChIP-seq]

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Transcription profiling of polysomal mRNA populations of mouse oocytes and zygotes

Project description:Transcriptional activation in mammalian embryos occurs in a stepwise manner. In mice, it begins at the late one-cell stage, followed by a minor wave of activation at the early two-cell stage, and then the major genome activation (MGA) at the late two-cell stage. Cellular homeostasis, metabolism, cell cycle, and developmental events are orchestrated before MGA by time-dependent changes in the array of maternal transcripts being translated (i.e., the translatome). Despite the importance of maternal mRNA and its correct recruitment for development, neither the array of recruited mRNA nor the regulatory mechanisms operating have been well cheracterized. We present the first comprehensive analysis of changes in the maternal component of the zygotic translatome during the transition from oocyte to late one-cell stage embryo, revealing global transitions in the functional classes of translated maternal mRNAs, and apparent changes in the underlying cis-regulatory mechanisms. Batches of 160-500 MII oocytes or one-cell embryos. 4 independent replicates per sample kind. Lysed and processed for polysomal mRNA extraction. Two rounds of IVT.

2007-12-07 | E-GEOD-3962 | biostudies-arrayexpress

Gene expression analysis of an endometrial cell line

Project description:This group is studying the role of glycoproteins in embryo implantation and development of the maternal-fetal interface. The Aplin lab is developing an embryo implantation model in which blastocysts attach to human endometrial cells. The project aims to investigate the molecular interactions mediating attachment and subsequent events including trophoblast invasion and displacement of maternal cells. In the present phase of the project, the lab is using the Ishikawa (human endometrial) cell line with mouse embryos.

2011-04-29 | GSE28925 | GEO

Mouse oocyte and one-cell embryo polysomal mRNA

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