Dataset Information

MSMS Data of Chymotrypsin digested Sublancin comprising fragments 12-32

ABSTRACT: Paper title: Sublancin is not a lantibiotic but an S-linked Glycopeptide. Author List: Oman TJ, Boettcher JM, Wang H, Okalibe XN, van der Donk WA. Citation: 60 citing articles. PubMedID: 21196935. Brief description of the data submitted: RAW Files used to generate Figure 1c and 1d in the main article.Fragmentation pattern of the chymotrypsin digest fragment comprising residues 12-32 of Sublancin

INSTRUMENT(S): Synapt MS

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168 (ncbitaxon:224308)

SUBMITTER: Wilfred A van der Donk

PROVIDER: MSV000079638 | MassIVE | Wed Apr 06 15:39:00 BST 2016

REPOSITORIES: MassIVE

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:In 15 patients with rheumatoid arthritis the baseline expression (t=0) of type I IFN-regulated genes was determined in peripheral blood cells (PAXgene) using cDNA-microarrays and compared to levels one month after (t=1) anti-TNF treatment (infliximab). Therefore, we used 43K cDNA microarrays from the Stanford Functional Genomics Facility (http://microarray.org/sfgf/) printed on aminosilane-coated slides containing ~20.000 unique genes. Only one batch of arrays was used for all experiments. First DNA spots were UV-cross linked to the slide using 150-300 mJoules. Sample preparation and microarray hybridization were performed as described previously (1,2). Data storage and filtering was performed using the Stanford Microarray Database (http://genome-www5.stanford.edu//) as described previously. 1. van der Pouw Kraan TC, Wijbrandts CA, van Baarsen LG, Voskuyl AE, Rustenburg F, Baggen JM et al.: Rheumatoid arthritis subtypes identified by genomic profiling of peripheral blood cells: assignment of a type I interferon signature in a subpopulation of patients. Ann Rheum Dis 2007, 66: 1008-1014. 2. van der Pouw Kraan TC, van Baarsen LG, Rustenburg F, Baltus B, Fero M, Verweij CL: Gene expression profiling in rheumatology. Methods Mol Med 2007, 136: 305-327. Abbreviations: - Bnrs are the different patients analyzed - PAX or Paxgene indicates the total RNA was isolated from peripheral blood obtained in PAXgene tubes - CRS: common reference sample: We made a common reference that consisted of a mixture of mRNAs isolated from 11 different cell lines (Perou CM, Jeffrey SS, van de RM et al. Distinctive gene expression patterns in human mammary epithelial cells and breast cancers. Proc Natl Acad Sci U S A. 1999; 96:9212-9217) supplemented with RNA from rheumatoid synovial tissue, fibroblasts, and activated peripheral blood mononuclear cells (PBMCs). - PMT: photomultiplier tube: the sensitivity/intensity of the scanner can be adjusted to prevent spot saturation. Compound Based Treatment: anti-TNF (infliximab)

Dataset Information

MSMS Data of Chymotrypsin digested Sublancin comprising fragments 12-32

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets