Dataset Information

Inner ear hair cell proteome

ABSTRACT: The mammalian inner ear subserves auditory and vestibular sensations via highly specialized cells and proteins. We show that sensory hair cells (HCs) employ hundreds of uniquely or highly expressed proteins for processes involved in transducing mechanical inputs, stimulating sensory neurons, and maintaining structure and function of these post-mitotic cells. Our proteomic analysis of purified HCs extends the existing HC transcriptome, revealing undetected gene products and isoform-specific protein expression. Comparison with mouse and human databases of genetic auditory/vestibular impairments confirms the critical role of the HC proteome for normal inner ear function, providing a cell-specific pool of candidates for novel, important HC genes. Several proteins identified exclusively in HCs by proteomics and by immunohistochemistry map to human genetic deafness loci, potentially representing new deafness genes.

INSTRUMENT(S): LTQ Velos, Orbitrap Fusion, LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Jeffrey Savas

PROVIDER: MSV000079756 | MassIVE | Mon May 23 10:42:00 BST 2016

SECONDARY ACCESSION(S): PXD004210

REPOSITORIES: MassIVE

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Publications

Global Analysis of Protein Expression of Inner Ear Hair Cells.

Hickox Ann E AE Wong Ann C Y AC Pak Kwang K Strojny Chelsee C Ramirez Miguel M Yates John R JR Ryan Allen F AF Savas Jeffrey N JN

The Journal of neuroscience : the official journal of the Society for Neuroscience 20161230 5

The mammalian inner ear (IE) subserves auditory and vestibular sensations via highly specialized cells and proteins. Sensory receptor hair cells (HCs) are necessary for transducing mechanical inputs and stimulating sensory neurons by using a host of known and as yet unknown protein machinery. To understand the protein composition of these unique postmitotic cells, in which irreversible protein degradation or damage can lead to impaired hearing and balance, we analyzed IE samples by tandem mass s ...[more]

PMID: 28039372

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