Proteomics

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Advancing top-down analysis of the human proteome using a benchtop quadrupole-Orbitrap mass spectrometer


ABSTRACT: Large scale study on human fibroblast, IMR90, by top-down proteomics on a benchtop Orbitrap QExactive HF instrument. RAW files were collected in three main modes: (i) data-dependent top-2 with high resolution for both MS1 and MS2, or hi/hi; (ii) in hi/hi using the in-house written instrument control software AUTOPILOT; (iii) in med/hi mode, where MS1 scans are acquired with short transients.

INSTRUMENT(S): Q Exactive HF (Thermo Scientific)

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Neil L. Kelleher 

PROVIDER: MSV000079913 | MassIVE | Fri Jul 15 19:09:00 BST 2016

REPOSITORIES: MassIVE

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