Proteomics

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Interactome analysis of PYHIN immune regulators

ABSTRACT: The human PYHIN proteins, AIM2, IFI16, IFIX, and MNDA, are regulators of immune response, transcription, apoptosis, and cell cycle. However, their protein interactions and underlying mechanisms remain largely uncharacterized. Here, we provide the interaction network for all PYHIN proteins. By designing a cell-based inducible system and integrating microscopy, immunoaffinity capture, quantitative mass spectrometry, and bioinformatics, we identified over 300 PYHIN interactions reflective of diverse functions.

OTHER RELATED OMICS DATASETS IN: PXD001572

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Ileana M Cristea 

PROVIDER: MSV000079955 | MassIVE | Wed Jul 20 12:00:00 BST 2016

SECONDARY ACCESSION(S): PXD001572

REPOSITORIES: MassIVE

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Publications

The functional interactome of PYHIN immune regulators reveals IFIX is a sensor of viral DNA.

Diner Benjamin A BA   Li Tuo T   Greco Todd M TM   Crow Marni S MS   Fuesler John A JA   Wang Jennifer J   Cristea Ileana M IM  

Molecular systems biology 20150209 1

The human PYHIN proteins, AIM2, IFI16, IFIX, and MNDA, are critical regulators of immune response, transcription, apoptosis, and cell cycle. However, their protein interactions and underlying mechanisms remain largely uncharacterized. Here, we provide the interaction network for all PYHIN proteins and define a function in sensing of viral DNA for the previously uncharacterized IFIX protein. By designing a cell-based inducible system and integrating microscopy, immunoaffinity capture, quantitativ  ...[more]