Proteomics

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Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex, and the Regulation of Transcription


ABSTRACT: MedPIC: Crosslinking-MS of 54 subunit pre-initiation complex from yeast. MedPolII: Crosslinking-MS of 33 subunit Mediator + polII complex from yeast.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Roger Kornberg 

PROVIDER: MSV000080013 | MassIVE | Tue Aug 02 13:43:00 BST 2016

REPOSITORIES: MassIVE

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Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.

Robinson Philip J PJ   Trnka Michael J MJ   Bushnell David A DA   Davis Ralph E RE   Mattei Pierre-Jean PJ   Burlingame Alma L AL   Kornberg Roger D RD  

Cell 20160901 6


A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, co  ...[more]

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