Dataset Information

Comprehensive Identification of Phosphorylation Sites on the Numb Endocytic Adaptor Protein

ABSTRACT: Numb is an endocytic adaptor protein that functions in the endocytosis and intracellular trafficking of membrane receptors and adhesion molecules. Numb localization and function have been shown to be regulated by phosphorylation through atypcial protein kinase C at several key sites. Here, we use Liquid Chromatogrpahy, Tandem Mass Spectrometry to identify additional Numb phosphorylation sites not yet characterized.

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Jane McGlade

PROVIDER: MSV000080218 | MassIVE | Tue Oct 04 11:57:00 BST 2016

SECONDARY ACCESSION(S): PXD000997

REPOSITORIES: MassIVE

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Comprehensive identification of phosphorylation sites on the Numb endocytic adaptor protein.

Krieger Jonathan R JR Taylor Paul P Moran Michael F MF McGlade C Jane CJ

Proteomics 20150101 2-3

Numb is an adaptor protein that functions in the endocytosis and intracellular trafficking of membrane receptors and adhesion molecules. Previous studies have indicated that Numb localization and function are regulated through phosphorylation by atypical protein kinase C at several key sites. Here, using LC-MS/MS, we report the identification of 25 serine/threonine Numb phosphorylation sites, and a single tyrosine phosphorylation site. Amino acid sequences flanking several of the sites identifie ...[more]

PMID: 25403733

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