A human interactome in three quantitative dimensions organized by stoichiometries and abundances -- auxiliary dataset

ABSTRACT: The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins under near-endogenous control, which we used as input for a next-generation interaction survey. Using quantitative proteomics, we detect specific interactions, estimate interaction stoichiometries and measures cellular abundances of interacting proteins. These three quantitative dimensions reveal that the protein network is dominated by weak, substoichiometric interactions that play a pivotal role in defining network topology. The minority of stable complexes can be identified by their unique stoichiometry signature. This study provides a rich interaction dataset connecting thousands of proteins, and introduces a framework for quantitative network analysis. -- This is an auxiliary dataset containing follow-up experiments. The main dataset is available under the identifier PXD002815.

INSTRUMENT(S): LTQ Orbitrap, Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) Mus Musculus (ncbitaxon:10090)

SUBMITTER: Dr Matthias Mann 

PROVIDER: MSV000080271 | MassIVE | Fri Oct 21 17:30:00 BST 2016

SECONDARY ACCESSION(S): PXD002829

REPOSITORIES: MassIVE