Proteomics

Dataset Information

0

IPG Strip-Based Peptide Fractionation for Shotgun Proteomics

ABSTRACT: This deep proteome dataset of human HEK293 cells was generated using IPG Strip-Based fractionation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Prof. Dr. Matthias Selbach 

PROVIDER: MSV000080274 | MassIVE | Fri Oct 21 21:15:00 BST 2016

SECONDARY ACCESSION(S): PXD002389

REPOSITORIES: MassIVE

Json Xml

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications

IPG strip-based peptide fractionation for shotgun proteomics.

Eravci Murat M   Sommer Christian C   Selbach Matthias M  

Methods in molecular biology (Clifton, N.J.) 20140101

Efficient fractionation of peptides is an essential prerequisite for comprehensive analysis of complex protein mixtures by shotgun mass spectrometry. The separation of peptides by isoelectric focusing is particularly attractive due to its orthogonality to reverse-phase HPLC. Here, we present a protocol for in-gel peptide isoelectric focusing using immobilized pH gradient strips. The method shows high resolving power for up to 1 mg of sample and is highly reproducible. ...[more]