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Proteomics of cerebrospinal fluid microvesicles

ABSTRACT: Comprehensive proteomic dataset of cerebrospinal fluid (CSF) microvesicles. The aim of this study is to deeply characterize the protein content of microvesicles (MVs) isolated from human cerebrospinal fluid. MVs were isolated from Two CSF pools and their proteome was studied with high sensitivity mass spectrometry, electron microscopy and western blot.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: None Listed

PROVIDER: MSV000080277 | MassIVE | Sun Oct 23 14:00:00 BST 2016

SECONDARY ACCESSION(S): PXD000608

REPOSITORIES: MassIVE

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Publications

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.

Chiasserini Davide D van Weering Jan R T JR Piersma Sander R SR Pham Thang V TV Malekzadeh Arjan A Teunissen Charlotte E CE de Wit Heidi H Jiménez Connie R CR

Journal of proteomics 20140424

Extracellular vesicles (EVs) are present in human cerebrospinal fluid (CSF), yet little is known about their protein composition. The aim of this study is to provide a comprehensive analysis of the proteome of CSF EVs by electron microscopy and high resolution tandem mass spectrometry (MS/MS) in conjunction with bioinformatics. We report an extensive catalog of 1315 proteins identified in EVs isolated from two different CSF pools by ultracentrifugation, including 230 novel EV proteins. Out of 13 ...[more]

PMID: 24769233

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Proteomics of cerebrospinal fluid microvesicles

Project description:Comprehensive proteomic dataset of cerebrospinal fluid (CSF) microvesicles. The aim of this study is to deeply characterize the protein content of microvesicles (MVs) isolated from human cerebrospinal fluid. MVs were isolated from Two CSF pools and their proteome was studied with high sensitivity mass spectrometry, electron microscopy and western blot.

2014-05-07 | PXD000608 | Pride

Human cerebrospinal fluid autoantibody lipid microarray profiling

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Phosphoproteome Analysis of Cerebrospinal Fluid Extracellular Vesicles in Primary Central Nervous System Lymphoma

Project description:Primary central nervous system lymphoma(PCNSL) is a rare extra-nodal non-Hodgkin’s lymphoma and accounts for 3%-4% of central nervous system tumors. Recent studies have highlighted the importance of cerebrospinal fluid derived extracellular vesicles in PCNSL. Extracellular vesicles(EVs) are nanoscale vesicles with bilayer lipid membrane released by almost all cell types. EVs are present in body fluids, including urine, blood and CSF. Cerebrospinal fluid(CSF) is a colorless fluid that surrounds the brain and spinal cord and acts as lymph in the central nervous system. CSF-derived EVs contain proteins from neurons, oligodendrocytes, astrocytes and microglias. Studies of CSF EVs are mainly limited by the amount of EVs isolated from per milliliter of CSF and the volume of CSF acquired from one patient. Here, we provide a label-free quantitative phospho-proteome profiling of EVs separated from PCNSL and non-PCNSL CSF samples by an earlier introduced functional magnetic beads called EVTRAP together with highly sensitive timsTOF Pro.

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Human cerebrospinal fluid autoantibody lipid microarray profiling (Fig. 1A)

Project description:Lipids comprise 70% of the myelin sheath, and autoantibodies against lipids may contribute to the demyelination that characterizes multiple sclerosis (MS). We used lipid antigen microarrays and lipid mass spectrometry to identify bona fide lipid targets of the autoimmune response in MS brain and an animal model of MS to explore the role of the identified lipids in autoimmune demyelination. We found that autoantibodies in MS target a phosphate group in phosphatidylserine and oxidized phosphatidylcholine derivatives. Administration of these lipids ameliorated experimental autoimmune encephalomyelitis by suppressing activation and inducing apoptosis of autoreactive T cells, effects mediated by the lipids' saturated fatty-acid side chains. Thus, phospholipids represent a natural anti-inflammatory class of compounds that have potential as novel therapeutics for MS. Fig. 1A. Lipid-array profiling of IgG+IgM antibody reactivity in cerebrospinal fluid (CSF) samples from MS patients (relapsing remitting MS; secondary progressive MS; primary progressive MS), healthy controls, and other neurological disease controls. Lipid hits with the lowest FDR (q=0.048) were clustered according to their reactivity profiles. 48 different lipids were custom-spotted in duplicate using the CAMAG Automatic TLC Sampler (ATS4) robot to spray 200 nl of 10 to 100 pmol of lipids onto PVDF membranes affixed to the surface of microscope slides. The slides were probed with cerebrospinal fluid (CSF) from 59 human patient samples. 60 slides total: 18 relapsing-remitting MS, 14 secondary-progressive MS, 1 primary-progressive MS, 21 other neurological disease, 5 healthy control, 1 secondary Ab alone (not included in this submission). CSF diluted 1/10. HRP-conjugated secondary Ab (goat anti-human IgM/IgG) diluted 1/8000. ECL for 3 minutes.

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Human Cerebrospinal Fluid modulates pathways promoting Glioblastoma malignancy

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Transcriptional profiling of cerebrospinal fluid cells of multiple sclerosis patients

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