Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-resolved metaproteomic characterization of preterm infant gut microbiota development reveals core metabolic functions and variability during early-life

ABSTRACT: An integrated metagenomics and metaproteomics investigation of human preterm infant gut microbiota.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Gut Microbiota Of Human Preterm Infants

SUBMITTER: Robert L. Hettich

PROVIDER: MSV000080565 | MassIVE | Thu Feb 23 08:35:00 GMT 2017

SECONDARY ACCESSION(S): PXD005962

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Publications

Genome-resolved metaproteomic characterization of preterm infant gut microbiota development reveals species-specific metabolic shifts and variabilities during early life.

Xiong Weili W Brown Christopher T CT Morowitz Michael J MJ Banfield Jillian F JF Hettich Robert L RL

Microbiome 20170710 1

<h4>Background</h4>Establishment of the human gut microbiota begins at birth. This early-life microbiota development can impact host physiology during infancy and even across an entire life span. However, the functional stability and population structure of the gut microbiota during initial colonization remain poorly understood. Metaproteomics is an emerging technology for the large-scale characterization of metabolic functions in complex microbial communities (gut microbiota).<h4>Results</h4>We ...[more]

PMID: 28693612

Similar Datasets

Metaproteome of preterm infant gut microbiome

Project description:91 preterm infant gut metaproteomes measured in technical duplicate using an eleven salt pulse 2D-LC-MS/MS method. Samples represent 17 preterm infants over the first several weeks of life, of which 6 preterm infants eventually developed necrotizing enterocolitis.

| MSV000086096 | MassIVE

Antibiotic resistance within the preterm infant gut.

Project description:The antibiotic resistance potential of the preterm infant gut microbiome measured using shotgun metagenomics.

| PRJEB15257 | ENA

Metaproteomics on preterm infant stool

Project description:Development of the gut microbiota is greatly impacted in preterm infants. Despite increasing knowledge about microbiota composition in preterm infants, knowledge about the functional signatures of the intestinal microbiota remains limited. The aim was to study transitions in microbiota activity during the first six postnatal weeks in ten preterm infants. A total of 64 stool samples were measured by LC-MS/MS.

2017-07-14 | PXD005574 | Pride

Preterm infant stool metagenomes

Project description:Longitudinal stool metagenomics from preterm infants within the first month after birth

| PRJEB52938 | ENA

Infant stool

Project description:Preterm infant stool metagenome

| PRJNA721684 | ENA

A Dysregulated Proinflammatory Status in Preterm School-aged Children with PVL-induced Spastic Cerebral Palsy

Project description:Injury occurring during critical periods of development may have long-term effects on inflammatory responses. Periventricular leukomalacia (PVL) is the most common cause of cerebral palsy (CP) in preterm infant. Activated leukocytes are the main source of inflammatory cytokines that give rise to white matter damage and CP in preterm infant. Here, we tested the hypothesis that inflammation profiles as pathogenic mediators for the occurrence of PVL in the neonatal period may persist in preterm children with CP at school age. Five preterm children with PVL-induced CP and gestational age-matched five preterm children with normal neurodevelopment were recruited from follow up clinics. Proinflammatory gene expression in the PBMCs from preterm children were determined by Superarray PCR study.

2010-05-22 | E-GEOD-17390 | biostudies-arrayexpress

Preterm infant intestinal microbiota

Project description:Development of the intestinal microbiota in preterm infants during the first six postnatal weeks determined by 454 pyrosequencing of the 16S rRNA gene.

| PRJEB18915 | ENA

Metaproteomic analysis of the infant fecal microbiome

Project description:A metaproteomics analysis was conducted on the infant fecal microbiome to characterize global protein expression in 8 samples obtained from infants with a range of early-life experiences. Samples included breast-, formula- or mixed-fed, mode of delivery, and antibiotic treatment and one set of monozygotic twins. Although label-free mass spectrometry-based proteomics is routinely used for the identification and quantification of thousands of proteins in complex samples, the metaproteomic analysis of the gut microbiome presents particular technical challenges. Among them: the extreme complexity and dynamic range of member taxa/species, the need for matched, well-annotated metagenomics databases, and the high inter-protein sequence redundancy/similarity between related members. In this study, a metaproteomic approach was developed for assessment of the biological phenotype and functioning, as a complement to 16S rRNA sequencing analysis to identify constituent taxa. A sample preparation method was developed for recovery and lysis of bacterial cells, followed by trypsin digestion, and pre-fractionation using Strong Cation Exchange chromatography. Samples were then subjected to high performance LC-MS/MS. Data was searched against the Human Microbiome Project database, and a homology-based meta-clustering strategy was used to combine peptides from multiple species into representative proteins. Bacterial taxonomies were also identified, based on species-specific protein sequences, and protein metaclusters were assigned to pathways and functional groups. The results obtained demonstrate the applicability of this approach for performing qualitative comparisons of human fecal microbiome composition, physiology and metabolism, and also provided a more detailed assessment of microbial composition in comparison to 16S rRNA.

2019-03-29 | PXD006033 | Pride

Peptidomics analysis of milk protein-derived peptides released over time in the preterm infant stomach

Project description:Over the course of milk digestion, native milk proteases and infant digestive proteases fragment intact proteins into peptides with potential bioactivity. This study investigated the release of peptides over three hours of gastric digestion in 14 preterm infant sample sets. The peptide content was extracted and analyzed from milk and gastric samples via Orbitrap tandem mass spectrometry. The relative ion intensity (abundance) and count of peptides in each sample were compared over time and between infants fed milk fortified with bovine milk fortifier and infants fed unfortified milk. Bioactivity of the identified peptides was predicted by sequence homology to known bioactive milk peptides. Both total and bioactive peptide abundance and count continuously increased over three hours of gastric digestion. After accounting for infant weight, length, and post-conceptual age, fortification of milk limited the release of peptides from human milk proteins. Peptides that survived further gastric digestion after their initial release were structurally more similar to bioactive peptides than non-surviving peptides. This work is the first to provide a comprehensive profile of milk peptides released during gastric digestion over time, which is an essential step in determining which peptides are most likely to be biologically relevant in the infant.

2019-03-12 | PXD012192 | Pride

Metaproteomics on preterm infant stool

2017-10-17 | MSV000081616 | MassIVE