Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of water emersion on the gill proteome of Mangrove killifish

ABSTRACT: Gill proteome of Mangrove killifish was compared by LC-MS/MS using 6 biological replicates of fish exposed to emersion stress versus 6 biological replicates kept under water that served as controls. PI: Experiments and fish exposures were performed in the laboratory of Prof. Patricia Wright at the University of Guelph (Canada). Proteomics contact: Proteomics sample preparation and biological mass spectrometry were performed in the laboratory of Prof. Dietmar Kueltz laboratory at UC Davis (CA).

INSTRUMENT(S): ImpactHD (Bruker Daltonics)

ORGANISM(S): Kryptolebias Marmoratus (ncbitaxon:37003)

SUBMITTER: Patricia Wright Dietmar Kueltz

PROVIDER: MSV000080634 | MassIVE | Wed Mar 15 14:57:00 GMT 2017

SECONDARY ACCESSION(S): PXD006100

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Publications

Skeletal stiffening in an amphibious fish out of water is a response to increased body weight.

Turko Andy J AJ Kültz Dietmar D Fudge Douglas D Croll Roger P RP Smith Frank M FM Stoyek Matthew R MR Wright Patricia A PA

The Journal of experimental biology 20171001 Pt 20

Terrestrial animals must support their bodies against gravity, while aquatic animals are effectively weightless because of buoyant support from water. Given this evolutionary history of minimal gravitational loading of fishes in water, it has been hypothesized that weight-responsive musculoskeletal systems evolved during the tetrapod invasion of land and are thus absent in fishes. Amphibious fishes, however, experience increased effective weight when out of water - are these fishes responsive to ...[more]

PMID: 29046415

Similar Datasets

Delayed effect of early-life exposure to 17-alpha-ethinylestradiol on brain proteome of adult mangrove rivulus fish

Project description:PI: Experiments and fish exposures were performed in the laboratory of Frederic Silvestre at the University of Namur (Belgium). Proteomics contact: Proteomics sample preparation and biological mass spectrometry were performed in the laboratory of Prof. Dietmar Kueltz at UC Davis (CA). LC-MS/MS data obtained from brains of 17 adult mangrove rivulus exposed to different doses of 17-alpha-ethinylestradiol during early development.

2018-08-13 | MSV000082814 | MassIVE

Salinity-dependent proteome dynamics in Nile tilapia gills

Project description:Experiments and fish acclimations were performed in the laboratory of Prof. Avner Cnaani at the Agricultural Research Organization (ARO) Volcani Center (Israel). Proteomics sample preparation and biological mass spectrometry analyses (DDA) were performed in the laboratory of Prof. Dietmar Kueltz at UC Davis (CA). Samples represent protein extracts from gill epithelium of individual fish. Proteins were extracted, digested, and prepared for online LCMS2 as described previously (Kultz et al., 2013, Molecular & Cellular Proteomics 12/12, 3962-3975). Each sample represents a different biological replicate obtained from a separate fish.

2020-06-27 | MSV000085639 | MassIVE

Salinity-dependent regulation of the kidney proteome of Oreochromis niloticus

Project description:PI: Experiments and fish acclimations were performed in the laboratory of Prof. Avner Cnaani at the Agricultural Research Organization (ARO) Volcani Center (Israel). Proteomics contact: Proteomics sample preparation and biological mass spectrometry analyses were performed in the laboratory of Prof. Dietmar Kueltz at UC Davis (CA). Database for spectral annotation: NCBI-Refseq.

2020-06-27 | MSV000085637 | MassIVE

The kidney proteome of the rufous-collared sparrow (Andean sparrow, Zonotrichia capensis) from Chile

Project description:Kidney proteomes of Rufous-collared sparrows from two different areas of the Atacama desert (Chile) were identified and compared by bottom-up proteomics utilizing nanoUPLC coupled online to tandem mass spectrometry. All experiments and sample collections were performed by the team of Dr. Paulina Gonzalez-Gomez. Proteomics sample preparation and biological mass spectrometry were performed in the laboratory of Prof. Dietmar Kueltz at UC Davis.

2018-04-26 | MSV000082314 | MassIVE

The plasma proteome of Rufous-collared sparrows (Zonotrichia capensis) from valleys of the Atacama Desert (Chile)

Project description:The plasma proteome of sparrows from the Atacama desert was identified using bottom up proteomics by online nanoLC tandem mass spectrometry. Seasonal and geographic effects on the plasma proteome were investigated. All experiments and sample collections were performed by the team of Dr. Paulina Gonzalez-Gomez. Proteomics sample preparation and biological mass spectrometry were performed in the laboratory of Prof. Dietmar Kueltz at UC Davis

2018-04-26 | MSV000082313 | MassIVE

Delayed effect of early-life exposure to 17-alpha-ethinylestradiol on the liver proteome of adult mangrove rivulus fish

Project description:LC-MS/MS data obtained from livers of 17 adult mangrove rivulus exposed to different doses of 17-alpha-ethinylestradiol during early development. All fish acclimations, treatments, experimental design, and data anlyses were performed in Frederic Silvestre's laboratory at the University of Namur, Belgium by Anne-Sophie Voisin. LC-MS/MS runs were done in Dietmar Kueltz's laboratory at UC Davis.

2018-08-13 | MSV000082815 | MassIVE

Delayed effect of early-life exposure to 17-alpha-ethinylestradiol on the gonad proteome of adult mangrove rivulus fish

Project description:LC-MS/MS data obtained from gonads of 17 adult mangrove rivulus exposed to different doses of 17-alpha-ethinylestradiol during early development. All fish acclimations, treatments, experimental design, and data anlyses were performed in Frederic Silvestre's laboratory at the University of Namur, Belgium by Anne-Sophie Voisin. LC-MS/MS runs were done in Dietmar Kueltz's laboratory at UC Davis.

2018-08-13 | MSV000082816 | MassIVE

Gene expression in liver, gill, hypothalamus, pituitary, and olfactory rosettes from Atlantic salmon parr and smolt

Project description:To identify genes involved in the developmental process of Atlantic salmon smoltification, gene expression was compared between smolt and parr in tissues involved in osmoregulation (gill), metabolism (liver), imprinting (olfactory rosettes) and neuroendocrine control (hypothalamus and pituitary). Tissue samples were harvested from laboratory-reared parr and smolts on the same date. Smolts were distinguished from parr by size and appearance; developmental status was confirmed by physiological assays. Eight biological replicates (16 fish) balanced for sex and for dye were used in the liver, gill, olfactory rosette, and hypothalamus comparisons. Four male parr were compared to four male smolts and four female parr were compared to four female smolts; smolts were labeled with Alexa Fluor 555 on four arrays and with Alexa Fluor 647 on four arrays. Six biological replicates (12 fish) were used for the pituitary comparison (two female and four male).

2012-08-20 | E-GEOD-33711 | biostudies-arrayexpress

Global gene expresssion profiling the gills of amoebic gill disease (AGD) affected Atlantic salmon (Salmo salar)

Project description:Amoebic gill disease (AGD) is an ectoparasitic condition of some farm-reared marine fish and is caused by Neoparamoeba perurans. Tanks housing Atlantic salmon (Salmo salar) were inoculated with Neoparamoeba perurans and fish were sampled at 36 days postinoculation (pi.). AGD-affected gill tissue was dissected from N. perurans infected fish, and a DNA microarray was used to compare global gene expression against tissues from AGD-naive fish. To determine whether the changes in gene expression were restricted to AGD-lesions, lesion tissue from AGD-affected fish was also compared with non-lesion gill tissue dissected from the same gill arch. Samples were assessed using a DNA microarray. mRNA from lesion and non-lesion gill tissue was amplified and labeled. Six biological and two technical replicates were utilised to hybridise to 12 arrays using amplified RNA from AGD-affected lesion gill tissue with AGD-naive fish as a control. Four biological and two technical replicates were utilised to hybridise to 8 arrays using amplified RNA from AGD-affected lesion gill tissue with non-lesion tissue from the same gill arch as a control. The assignment of microarrays to treatment groups for hybridization was randomised by using a random number generator.

2010-05-25 | E-GEOD-9595 | biostudies-arrayexpress

Transcriptome analysis of miRNA–mRNA interactions revealed regulation of multiple signaling pathways in genetically improved farmed tilapia (GIFT, Oreochromis niloticus) gill tissues under hypoxia stress

Project description:Dissolved oxygen (DO) is the basis of fish survival, and proper DO level is an important condition to ensure the normal growth of fish. Hypoxic environment is prone to disturb the normal breathing and metabolism of fish, which in turn affects their growth and survival. Gill tissue is the respiratory organs of fish and is in direct contact with the external environment. However, there are few reports on the molecular regulatory mechanism of genetically improved farmed tilapia (GIFT, Oreochromis niloticus) gill tissues in response to hypoxia. Here, we first examine the hypoxia-induced damage of gill tissue by hematoxylin-eosin staining, and then constructed miRNA and mRNA libraries of GIFT gill tissue at 96h of hypoxia stress by a high-throughput sequencing technology, each library has three biological replicates. Gill lamellae of GIFT showed capillary rupture and red blood cell enlargement and overflow under hypoxia stress. Transcription sequencing results showed that the clean reads of miRNA libraries were 9,627,953-13,544,660; the clean reads of mRNA libraries were 43,817,776-53,130,102. Based on the miRNA-mRNA pairs screening principles and mRNA sequencing results, we selected and verified seven differentially expressed miRNAs and their potential target genes. The sequencing results were consistent with the qRT-PCR validation results. These selected miRNA-mRNA pairs are mainly concentrated in the signaling pathways of immune response and metabolic regulation. This study provides new insights into the mechanisms of fish adaptation under hypoxic stress.

2023-02-22 | GSE145788 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data