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DeMix:efficient identification of co-fragmented peptides

ABSTRACT: A data analysis workflow with a simple deconvolution method based on high resolution data-dependent tandem mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Roman A. Zubarev

PROVIDER: MSV000080702 | MassIVE | Tue Mar 28 21:47:00 BST 2017

SECONDARY ACCESSION(S): PXD000999

REPOSITORIES: MassIVE

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DeMix workflow for efficient identification of cofragmented peptides in high resolution data-dependent tandem mass spectrometry.

Zhang Bo B Pirmoradian Mohammad M Chernobrovkin Alexey A Zubarev Roman A RA

Molecular & cellular proteomics : MCP 20140806 11

Based on conventional data-dependent acquisition strategy of shotgun proteomics, we present a new workflow DeMix, which significantly increases the efficiency of peptide identification for in-depth shotgun analysis of complex proteomes. Capitalizing on the high resolution and mass accuracy of Orbitrap-based tandem mass spectrometry, we developed a simple deconvolution method of "cloning" chimeric tandem spectra for cofragmented peptides. Additional to a database search, a simple rescoring scheme ...[more]

PMID: 25100859

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