Proteomics

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Proteomic analysis of glutamine deprivation in HepG2 cells


ABSTRACT: We applied the quantitative proteomics approach to estimate the relative abundance of proteins in HepG2 cells treated by glutamine deprivation. Two independent cultured cell lysates, namely control and glutamine deprivation, were marked with iTRAQ labels and subjected to LC-ESI-MS/MS analysis. The tryptic peptides of two samples were labeled with mass 113 (control) and 119 (glutamine deprivation) isobaric iTRAQ tags.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Xianghua Yan  

PROVIDER: MSV000080721 | MassIVE | Wed Mar 29 00:59:00 BST 2017

SECONDARY ACCESSION(S): PXD003387

REPOSITORIES: MassIVE

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Publications

Quantitative proteomics analysis reveals glutamine deprivation activates fatty acid β-oxidation pathway in HepG2 cells.

Long Baisheng B   Muhamad Rodiallah R   Yan Guokai G   Yu Jie J   Fan Qiwen Q   Wang Zhichang Z   Li Xiuzhi X   Purnomoadi Agung A   Achmadi Joelal J   Yan Xianghua X  

Amino acids 20160202 5


Glutamine, a multifunctional amino acid, functions in nutrient metabolism, energy balance, apoptosis, and cell proliferation. Lipid is an important nutrient and controls a broad range of physiological processes. Previous studies have demonstrated that glutamine can affect lipolysis and lipogenesis, but the effect of glutamine on the detailed lipid metabolism remains incompletely understood. Here, we applied the quantitative proteomics approach to estimate the relative abundance of proteins in He  ...[more]

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