Proteomics

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Interactome of Nematostella GW182 in HEK293 cells

ABSTRACT: Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translation and promoting mRNA decay. Further, similarly to bilaterians, GW182 in Nematostella is recruited to the miRNA repression complex via interaction with Argonaute proteins, and functions downstream to repress mRNA. Thus, our work suggests that this mechanism of miRNA-mediated silencing was already active in the last common ancestor of Cnidaria and Bilateria. Despite this remarkable mechanistic conservation Argonaute and GW182 differentially co-evolved in bilaterians and cnidarians seemingly leading to distinct interaction surfaces.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Matthias Selbach  

PROVIDER: MSV000080764 | MassIVE | Wed Mar 29 11:41:00 BST 2017

SECONDARY ACCESSION(S): PXD004668

REPOSITORIES: MassIVE

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Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution.

Mauri Marta M   Kirchner Marieluise M   Aharoni Reuven R   Ciolli Mattioli Camilla C   van den Bruck David D   Gutkovitch Nadya N   Modepalli Vengamanaidu V   Selbach Matthias M   Moran Yehu Y   Chekulaeva Marina M  

Nucleic acids research 20160906 2

Our current knowledge about the mechanisms of miRNA silencing is restricted to few lineages such as vertebrates, arthropods, nematodes and land plants. miRNA-mediated silencing in bilaterian animals is dependent on the proteins of the GW182 family. Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptoph  ...[more]

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