Proteomics

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Uncovering Global SUMOylation Signaling Networks in a Site-Specific Manner

ABSTRACT: SUMOylation is a reversible post-translational modification essential for genome stability. Using high-resolution mass spectrometry, we have studied global SUMOylation in mammalian cells and in a site-specific manner, identifying a total of over 4,300 SUMOylation sites in over 1,600 proteins. Moreover, for the first time in excess of 1,000 SUMOylation sites were identified under standard growth conditions. SUMOylation dynamics were quantitatively studied in response to SUMO protease inhibition, proteasome inhibition and heat shock.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Alfred C.O. Vertegaal  

PROVIDER: MSV000080793 | MassIVE | Wed Mar 29 15:56:00 BST 2017

SECONDARY ACCESSION(S): PXD001061

REPOSITORIES: MassIVE

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Uncovering global SUMOylation signaling networks in a site-specific manner.

Hendriks Ivo A IA   D'Souza Rochelle C J RC   Yang Bing B   Verlaan-de Vries Matty M   Mann Matthias M   Vertegaal Alfred C O AC  

Nature structural & molecular biology 20140914 10

SUMOylation is a reversible post-translational modification essential for genome stability. Using high-resolution MS, we have studied global SUMOylation in human cells in a site-specific manner, identifying a total of >4,300 SUMOylation sites in >1,600 proteins. To our knowledge, this is the first time that >1,000 SUMOylation sites have been identified under standard growth conditions. We quantitatively studied SUMOylation dynamics in response to SUMO protease inhibition, proteasome inhibition a  ...[more]