Proteomics

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Quest for missing proteins in the human spermatozoa: an update

ABSTRACT: Here, we report work as part of C-HPP initiatives on chromosomes 14 (France) and 2 (Switzerland) aiming to identify missing proteins in ejaculated human spermatozoa using multiple MS-based and antibodies-based validation approaches

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Christophe Bruley  

PROVIDER: MSV000080826 | MassIVE | Fri Mar 31 13:39:00 BST 2017

SECONDARY ACCESSION(S): PXD003947

REPOSITORIES: MassIVE

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The Chromosome-Centric Human Proteome Project (C-HPP) aims to identify "missing" proteins in the neXtProt knowledgebase. We present an in-depth proteomics analysis of the human sperm proteome to identify testis-enriched missing proteins. Using protein extraction procedures and LC-MS/MS analysis, we detected 235 proteins (PE2-PE4) for which no previous evidence of protein expression was annotated. Through LC-MS/MS and LC-PRM analysis, data mining, and immunohistochemistry, we confirmed the expres  ...[more]

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