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Large-Scale Targeted Proteomics Using Internal Standard Triggered-Parallel Reaction Monitoring

ABSTRACT: Development, implementation, and evaluation of a new data acquisition scheme called internal standard triggered-parallel reaction monitoring (IS-PRM) to increase the scale of targeted quantitative experiments while retaining high detection and quantification performance. All the details about the dataset, the associated sample preparation and liquid chromatography coupled to tandem mass spectrometry methods, and the data processing procedures are provided in the manuscript by Gallien et al., entitled "Large-Scale Targeted Proteomics Using Internal Standard Triggered-Parallel Reaction Monitoring", Molecular and Cellular Proteomics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Bruno Domon

PROVIDER: MSV000080832 | MassIVE | Fri Mar 31 17:03:00 BST 2017

SECONDARY ACCESSION(S): PXD001731

REPOSITORIES: MassIVE

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Large-Scale Targeted Proteomics Using Internal Standard Triggered-Parallel Reaction Monitoring (IS-PRM).

Gallien Sebastien S Kim Sang Yoon SY Domon Bruno B

Molecular & cellular proteomics : MCP 20150309 6

Targeted high-resolution and accurate mass analyses performed on fast sequencing mass spectrometers have opened new avenues for quantitative proteomics. More specifically, parallel reaction monitoring (PRM) implemented on quadrupole-orbitrap instruments exhibits exquisite selectivity to discriminate interferences from analytes. Furthermore, the instrument trapping capability enhances the sensitivity of the measurements. The PRM technique, applied to the analysis of limited peptide sets (typicall ...[more]

PMID: 25755295

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