Dataset Information

Online non-contiguous fractionating and concatenating device coupled to two dimensional reverse phase/reverse phase liquid chromatography system for efficient and comprehensive proteomic analyses

ABSTRACT: The goal of the project is development of an online two dimensional reverse phase/reverse phase liquid chromatography (2D-RP/RPLC) separation platform, employing an online non-contiguous fractionating and concatenating device (NCFC fractionator) in order to provide highly improve separation orthogonality of the two RPs, resulting in significant increase in peptide identification sensitivity compared with those of the conventional contiguous 2D-RP/RPLC method.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Sang-Won Lee

PROVIDER: MSV000080846 | MassIVE | Fri Mar 31 22:09:00 BST 2017

SECONDARY ACCESSION(S): PXD004710

REPOSITORIES: MassIVE

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Publications

Efficient Exploitation of Separation Space in Two-Dimensional Liquid Chromatography System for Comprehensive and Efficient Proteomic Analyses.

Lee Hangyeore H Mun Dong-Gi DG So Jeong Eun JE Bae Jingi J Kim Hokeun H Masselon Christophe C Lee Sang-Won SW

Analytical chemistry 20161111 23

Proteomics aims to achieve complete profiling of the protein content and protein modifications in cells, tissues, and biofluids and to quantitatively determine changes in their abundances. This information serves to elucidate cellular processes and signaling pathways and to identify candidate protein biomarkers and/or therapeutic targets. Analyses must therefore be both comprehensive and efficient. Here, we present a novel online two-dimensional reverse-phase/reverse-phase liquid chromatography ...[more]

PMID: 27801565

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