Proteomics

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Quantitative Proteome Analysis Reveals Increased Content of Basement Membrane Proteins in Arteries from Patients with Type 2 Diabetes and Lower Levels among Metformin Users

ABSTRACT: We analyzed non-atherosclerotic repair arteries gathered at coronary by-pass operations from 30 patients with type 2 diabetes, as well as from 30 age- and gender-matched non-diabetic individuals. Quantitative proteome analysis was done by iTRAQ-labelling and LC-MS/MS analysis on individual arterial samples. The amounts of the basement membrane (BM) components, alpha-1- and alpha-2- type IV collagen, gamma-1- and beta-2-laminin were significantly increased in patients with diabetes. Moreover, the expressions of basement membrane components and other vascular proteins were significantly lower among metformin users, as compared to non-users. Patients treated with or without metformin had similar levels of HbA1c, cholesterol and blood pressure. In addition, quantitative histomorphometry showed increased area fractions of collagen-stainable material in tunica intima and media among patients with diabetes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Hans Christian Beck  

PROVIDER: MSV000080848 | MassIVE | Fri Mar 31 22:29:00 BST 2017

SECONDARY ACCESSION(S): PXD002214

REPOSITORIES: MassIVE

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Quantitative Proteome Analysis Reveals Increased Content of Basement Membrane Proteins in Arteries From Patients With Type 2 Diabetes Mellitus and Lower Levels Among Metformin Users.

Preil Simone A R SA   Kristensen Lars P LP   Beck Hans C HC   Jensen Pia S PS   Nielsen Patricia S PS   Steiniche Torben T   Bjørling-Poulsen Marina M   Larsen Martin R MR   Hansen Maria L ML   Rasmussen Lars M LM  

Circulation. Cardiovascular genetics 20150914 5

<h4>Background</h4>The increased risk of cardiovascular diseases in type 2 diabetes mellitus has been extensively documented, but the origins of the association remain largely unknown. We sought to determine changes in protein expressions in arterial tissue from patients with type 2 diabetes mellitus and moreover hypothesized that metformin intake influences the protein composition.<h4>Methods and results</h4>We analyzed nonatherosclerotic repair arteries gathered at coronary bypass operations f  ...[more]

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