Proteomics

Dataset Information

0

Phosphoproteome of a patient-matched cell line model of colon cancer metastasis


ABSTRACT: The contributions of phosphorylation-mediated signaling networks to colon cancer metastasis are poorly defined. To better understand the role of constitutive signaling alterations in cancer progression, the global phosphoproteomes of the SW480 and SW620 cell lines were compared by LC-MS/MS. These patient-matched cell lines were derived from a primary colon tumor and a lymph node metastasis, respectively. Network analysis of the significantly altered phosphosites revealed differential regulation in cellular adhesion, mitosis, and mRNA translational machinery. Among the latter group were sites on phosphoproteins with known roles in mRNA biogenesis and splicing, transport through the nuclear pores, initiating translation, as well as mRNA stability and degradation. Though alterations in these processes have been associated with oncogenic transformation, control of mRNA stability has typically not been associated with cancer progression. Notably, the single phosphosite with the greatest relative change in SW620 was Ser2 on eIF2S2, suggesting that SW620 cells translate faster or with greater efficiency than SW480 cells. These broad changes in the regulation of translation also occur without over-expression of eIF4E. Altogether, this work shows that metastatic cells exhibit constitutive changes to the phosphoproteome, and that mRNA stability and translational efficiency may be important targets of deregulation during cancer progression.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Amanda B. Hummon  

PROVIDER: MSV000080864 | MassIVE | Sat Apr 01 01:54:00 BST 2017

SECONDARY ACCESSION(S): PXD003708

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications

Phosphoproteomics of colon cancer metastasis: comparative mass spectrometric analysis of the isogenic primary and metastatic cell lines SW480 and SW620.

Schunter Alissa J AJ   Yue Xiaoshan X   Hummon Amanda B AB  

Analytical and bioanalytical chemistry 20161216 7


The contributions of phosphorylation-mediated signaling networks to colon cancer metastasis are poorly defined. To interrogate constitutive signaling alterations in cancer progression, the global phosphoproteomes of patient-matched SW480 (primary colon tumor origin) and SW620 (lymph node metastasis) cell lines were compared with TiO<sub>2</sub> and immobilized metal affinity chromatography phosphopeptide enrichment followed by liquid chromatography-tandem mass spectrometry. Network analysis of t  ...[more]

Similar Datasets

2017-01-06 | PXD003708 | Pride
2020-07-23 | GSE154927 | GEO
2014-01-23 | E-GEOD-54299 | biostudies-arrayexpress
2020-07-23 | GSE154930 | GEO
2004-09-01 | GSE1323 | GEO
2016-08-01 | E-GEOD-72412 | biostudies-arrayexpress
2019-07-02 | GSE70458 | GEO
2009-02-20 | E-MEXP-2060 | biostudies-arrayexpress
2015-12-13 | GSE74862 | GEO
2008-06-13 | E-GEOD-5486 | biostudies-arrayexpress