Initiation of DNA replication requires actin dynamics and formin activity
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ABSTRACT: Label-free identification of the combined chromatin+insoluble proteome of nuclei formed in 50 minutes in Xenopus egg extracts containing DMSO (vehicle) or purvalanol A (cdk inhibitor). Nuclei were isolated by a differential centrifugation through sucrose cushion and lysed with Triton X100 (0.3%).
INSTRUMENT(S): LTQ Orbitrap XL
ORGANISM(S): Xenopus Laevis (ncbitaxon:8355)
SUBMITTER: Fischer
PROVIDER: MSV000081201 | MassIVE | Wed Jun 28 04:20:00 BST 2017
REPOSITORIES: MassIVE
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