Proteomics

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Multi-omics and Network Evaluation of Arogenate Dehydratase Knock-out Mutants in Arabidopsis thaliana

ABSTRACT: By knocking out genes encoding specific arogenate dehydrase (ADT) enzymes in Arabidopsis, variable reductions in lignin can be achieved. To understand how ADT composition affects plant phenotypes and biomolecular systems, we successfully constructed single and multiple ADT knockout (KO) mutants in Arabidopsis. Using these mutants, a multi-omics (metabolome, transcriptome and proteome) evaluation was conducted using GC- and LC-MS, RNA-Seq, and iTRAQ labeled LC-MS/MS technologies. Identifications include primary and secondary metabolites, transcripts, and proteins in leaf and stem samples taken at 4 weeks of age from 9 KO and wild-type (WT) lines.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Norman G. Lewis  

PROVIDER: MSV000081518 | MassIVE |

SECONDARY ACCESSION(S): PXD007701

REPOSITORIES: MassIVE

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New Insights Into Lignification via Network and Multi-Omics Analyses of Arogenate Dehydratase Knock-Out Mutants in <i>Arabidopsis thaliana</i>.

Hixson Kim K KK   Marques Joaquim V JV   Wendler Jason P JP   McDermott Jason E JE   Weitz Karl K KK   Clauss Therese R TR   Monroe Matthew E ME   Moore Ronald J RJ   Brown Joseph J   Lipton Mary S MS   Bell Callum J CJ   Paša-Tolić Ljiljana L   Davin Laurence B LB   Lewis Norman G NG  

Frontiers in plant science 20210525

Multiple <i>Arabidopsis</i> arogenate dehydratase (<i>ADT</i>) knock-out (KO) mutants, with phenotypes having variable lignin levels (up to <i>circa</i> 70% reduction), were studied to investigate how differential reductions in ADTs perturb its overall plant systems biology. Integrated "omics" analyses (metabolome, transcriptome, and proteome) of wild type (WT), single and multiple <i>ADT</i> KO lines were conducted. Transcriptome and proteome data were collapsed into gene ortholog (GO) data, wi  ...[more]

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