Proteomics

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Confetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics

ABSTRACT: Tryptic digestion proteomics gives limited coverage of many protein sequences as many proteins produce tryptic peptides that cannot be observed easily. Confetti is our comprehensive map of coverage for the HeLa proteome, using 48 different digests and CID/HCD LC-MS/MS analyses. An associated dataset belonging to the same study can be found under the PX identifier PXD001258.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Hamid Mirzaei  

PROVIDER: MSV000081607 | MassIVE | Thu Oct 12 03:06:00 BST 2017

SECONDARY ACCESSION(S): PXD000900

REPOSITORIES: MassIVE

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Confetti: a multiprotease map of the HeLa proteome for comprehensive proteomics.

Guo Xiaofeng X   Trudgian David C DC   Lemoff Andrew A   Yadavalli Sivaramakrishna S   Mirzaei Hamid H  

Molecular & cellular proteomics : MCP 20140402 6

Bottom-up proteomics largely relies on tryptic peptides for protein identification and quantification. Tryptic digestion often provides limited coverage of protein sequence because of issues such as peptide length, ionization efficiency, and post-translational modification colocalization. Unfortunately, a region of interest in a protein, for example, because of proximity to an active site or the presence of important post-translational modifications, may not be covered by tryptic peptides. Detec  ...[more]