Dataset Information

Differential Proteomic Analysis of Bordetella pertussis OMV

ABSTRACT: Differential proteomic analysis of outer membrane vesicles from Bordetella pertussis (two strains: Bvg+ and Bvg-)

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bordetella Pertussis (ncbitaxon:520)

SUBMITTER: Gianmarco Gasperini

PROVIDER: MSV000081702 | MassIVE | Thu Nov 09 06:50:00 GMT 2017

SECONDARY ACCESSION(S): PXD008179

REPOSITORIES: MassIVE

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Publications

Outer Membrane Vesicles (OMV)-based and Proteomics-driven Antigen Selection Identifies Novel Factors Contributing to Bordetella pertussis Adhesion to Epithelial Cells.

Gasperini Gianmarco G Biagini Massimiliano M Arato Vanessa V Gianfaldoni Claudia C Vadi Alessandro A Norais Nathalie N Bensi Giuliano G Delany Isabel I Pizza Mariagrazia M Aricò Beatrice B Leuzzi Rosanna R

Molecular & cellular proteomics : MCP 20171204 2

Despite high vaccination coverage world-wide, whooping cough, a highly contagious disease caused by Bordetella pertussis, is recently increasing in occurrence suggesting that novel vaccine formulations targeted at the prevention of colonization and transmission should be investigated. To identify new candidates for inclusion in the acellular formulation, we used spontaneously released outer membrane vesicles (OMV)1 as a potential source of key adhesins. The enrichment of Bvg+ O ...[more]

PMID: 29203497

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Project description:At present, the function of Fusobacterium nucleosocci outer membrane vesicles under normal intestinal environmental pH conditions has been mainly studied, but there are few studies related to the function of Fusobacterium nucleosius outer membrane vesicles under cancer microenvironment pH conditions. The purpose of this study was to pioneer the comparative analysis of the composition of Fusobacterium nucleosibacterium outer membrane vesicle proteins under normal intestinal environmental pH conditions and cancer microenvironment pH conditions, and to find out the virulence factor protein of Fusobacterium nucleosocci outer membrane vesicles under cancer microenvironment pH conditions. To do this, we adjusted the initial pH of TSPC medium to around 6.0 using acidic cysteine to mimic the cancer microenvironment pH conditions. We combined ultracentrifugation and density gradient centrifugation to separate and extract Fusobacterium nucleosi's outer membrane vesicles from two pH environments, and used TMT quantitative proteomics to identify proteins in outer membrane vesicles. A total of 991 proteins were identified, compared with normal outer membrane vesicles, 306 proteins in the outer membrane vesicles in the tumor environment were up-regulated and 360 proteins were down-regulated, and some of the up-regulated proteins of the out-membrane vesicles in the tumor environment were virulence factor proteins, such as multiple pentatype autotransporters and MORN2 domain proteins, and the functions of these proteins were mainly related to adhesion and invasion. In addition, the down-regulation of enzyme levels involved in butyrate metabolism in the outer membrane vesicles of Fusobacterium nucleosionae under the condition of cancer microenvironment pH inhibits the butyric acid production capacity of Fusobacterium nucleobacteria, resulting in a decrease in butyric acid production capacity, which may promote the development of colorectal cancer. In conclusion, quantitative proteomics comparative analysis has increased our understanding of Fusobacterium nucleosom outer membrane vesicle virulence protein in the tumor environment.

Dataset Information

Differential Proteomic Analysis of Bordetella pertussis OMV

Dataset's files

Publications

Outer Membrane Vesicles (OMV)-based and Proteomics-driven Antigen Selection Identifies Novel Factors Contributing to <i>Bordetella pertussis</i> Adhesion to Epithelial Cells.

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